Metabolic enhancement and increase of alveolar macrophages induced by ozone
Male Wistar rats were exposed to 0.2 ppm ozone (O3) for 14 days and at intervals alveolar macrophages were collected by bronchoalveolar lavage to examine the effects of O3. The specific activities of glucose-6-phosphate dehydrogenase and glutathione peroxidase of alveolar macrophages increased to 1.6-fold (on the 3rd day) and 1.5-fold (on the 5th day), respectively, those of the control values. Similarly, the specific activities of pyruvate kinase, lactate dehydrogenase, and hexokinase also increased to 1.6-fold, 1.4-fold, and 1.2-fold, respectively, those of the control values on the 3rd day. The activities of all enzymes tested were maintained at significantly higher levels until the 14th day. Furthermore, the incorporation of (14C)thymidine into alveolar macrophages increased twice the control values on the 1st and 3rd days and was almost completely inhibited by the addition of 1.23 x 10(-4) M aphidicolin, a competitive inhibitor of DNA polymerase alpha. The number of alveolar macrophages collected from exposed animals also increased to 1.5-fold that of the control value on the 3rd day and was maintained at significantly higher level until the 14th day. It was noted that alveolar macrophages of small size preferentially increased between the 5th and 14th days. These results show that exposures to 0.2 ppm O3 induced a metabolic enhancement of the peroxidative metabolism, glycolysis, and DNA synthesis in alveolar macrophages and increased the macrophages of small size.
- Research Organization:
- National Institute for Environmental Studies, Ibaraki (Japan)
- OSTI ID:
- 5926310
- Journal Information:
- Environ. Res.; (United States), Vol. 49:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OXIDOREDUCTASES
ENZYME ACTIVITY
OZONE
METABOLISM
PEROXIDASES
PHOSPHOTRANSFERASES
CARBON 14 COMPOUNDS
ENZYME INHIBITORS
GLUTATHIONE
LAVAGE
MACROPHAGES
RATS
THYMIDINE
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
AZINES
CONNECTIVE TISSUE CELLS
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHAGOCYTES
PHOSPHORUS-GROUP TRANSFERASES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RADIOPROTECTIVE SUBSTANCES
RIBOSIDES
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology