Accumulation of galactosylsphingosine (psychosine) does not interfere with phosphorylation and methylation of myelin basic protein in the twitcher mouse

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Neurochemical Research; (USA)

DOI:https://doi.org/10.1007/BF00965740· OSTI ID:5923788

Yoshimura, T; Kobayashi, T; Shinnoh, N; Goto, I ^[1]

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1. Kyushu Univ., Fukuoka (Japan)

In attempts to elucidate mechanisms of demyelination in the twitcher mouse (Twi), phosphorylation and methylation of myelin basic protein (MBP) were examined in the brainstem and spinal cord of this species. Phosphorylation of MBP in isolated myelin by an endogenous kinase and an exogenous (32P)ATP was not impaired and protein kinase C activity in the brain cytosol was not reduced. When the methylation of an arginine residue of MBP was examined in slices of the brainstem and spinal cord, using (3H)methionine as a donor of the methyl groups, no difference was found between Twi and the controls. Radioactivity of the (3H) methionine residue of MBP of Twi was also similar to that of the controls. Thus, accumulation of psychosine in Twi does not interfere with the activity of endogenous kinase, methylation of MBP, and the synthesis and transport of MBP into myelin membrane.

• OSTI ID: 5923788
• Journal Information: Neurochemical Research; (USA), Vol. 15:10; ISSN 0364-3190
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MYELIN
METABOLISM
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROTEINS
BIOLOGICAL ACCUMULATION
AC AMPLIFIERS
ARGININE
ATP
AUTORADIOGRAPHY
BRAIN
CELL MEMBRANES
METHIONINE
METHYLATION
MICE
PHOSPHORUS 32
PHOSPHORYLATION
SPINAL CORD
TRITIUM COMPOUNDS
AMINO ACIDS
AMPLIFIERS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRONIC EQUIPMENT
ENZYMES
EQUIPMENT
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
RODENTS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques