Cross-linking and modification of cytochrome c with redox-active metal complexes
Technical Report
·
OSTI ID:5923311
This thesis consists of two parts. The first part shows that a redox-active trinuclear metal cluster may be used as a cross-linking reagent for proteins. Electron transfer is observed in the protein oligomers. The second part involves labelling the cysteine residue of baker's yeast cytochrome c with chloromercuriferrocene. Chloromercuriferrocene reacts with cytochrome c in two interesting ways. Symmetrization produces two products; two proteins cross-linked with mercury and diferrocenylmercury. Simple substitution of FeHgCl onto the protein followed by the addition of a proton by electrophilic substitution affords ferrocene and the mercuric chloride modified protein. 16 refs., 3 figs.
- Research Organization:
- Ames Lab., IA (USA)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 5923311
- Report Number(s):
- IS-T-1522; ON: DE91012023
- Resource Relation:
- Other Information: Thesis (M.S.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CYTOCHROMES
CROSS-LINKING
ION PAIRS
CHEMISTRY
CHLORINE COMPOUNDS
COMPLEXES
CYSTEINE
ELECTRON TRANSFER
IRON COMPOUNDS
MERCURY COMPOUNDS
ORGANIC COMPOUNDS
REDOX PROCESS
YEASTS
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
EUMYCOTA
FUNGI
HALOGEN COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PLANTS
POLYMERIZATION
PROTEINS
REPROCESSING
SEPARATION PROCESSES
THIOLS
TRANSITION ELEMENT COMPOUNDS
550200* - Biochemistry
400201 - Chemical & Physicochemical Properties
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CYTOCHROMES
CROSS-LINKING
ION PAIRS
CHEMISTRY
CHLORINE COMPOUNDS
COMPLEXES
CYSTEINE
ELECTRON TRANSFER
IRON COMPOUNDS
MERCURY COMPOUNDS
ORGANIC COMPOUNDS
REDOX PROCESS
YEASTS
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
EUMYCOTA
FUNGI
HALOGEN COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PLANTS
POLYMERIZATION
PROTEINS
REPROCESSING
SEPARATION PROCESSES
THIOLS
TRANSITION ELEMENT COMPOUNDS
550200* - Biochemistry
400201 - Chemical & Physicochemical Properties