Prolactin-stimulated ornithine decarboxylase induction in rat hepatocytes: Coupling to diacylglycerol generation and protein kinase C
- Kirksville College of Osteopathic Medicine, MO (USA)
The trophic effects of prolactin (PRL) in rat liver have been linked to activation of protein kinase C (PKC). Since alterations in PKC activity imply its activation by 1,2-diacylglycerol (DAG), we tested whether PRL treatment stimulated DAG generation coupled to induction of a growth response in primary hepatocytes. Addition of PRL to hepatocyte cultures significantly increased ({sup 3}H)-glycerol incorporation into DAG within 5 minutes which was followed by a loss of cytosolic PKC activity by 10 minutes. Prolactin also significantly enhanced radiolabel incorporation into triacylglycerol and phospholipids within 10 minutes and induced ODC activity at 6 hours. Therefore, prolactin-stimulated alterations in PKC activity are preceded by enhanced DAG generation. Moreover, these events appear to be coupled to PRL-stimulated entry of hepatocytes into cell cycle.
- OSTI ID:
- 5922656
- Journal Information:
- Life Sciences; (USA), Vol. 48:3; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DECARBOXYLASES
ENZYME INDUCTION
GLYCEROL
BIOSYNTHESIS
LTH
BIOCHEMICAL REACTION KINETICS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CELL CYCLE
GROWTH
LIVER CELLS
ORNITHINE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
ENZYMES
GENE REGULATION
GONADOTROPINS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LYASES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PITUITARY HORMONES
REACTION KINETICS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques