Transport of indoleacetic acid in intact corn coleoptiles. [Zea mays L]

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.2.417· OSTI ID:5915108

Parker, K E; Briggs, W R ^[1]

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1. Carnegie Institution of Washington, Stanford, CA (USA)

We have characterized the transport of ({sup 3}H)indoleacetic acid (IAA) in intact corn (Zea mays L.) coleoptiles. We have used a wide range of concentrations of added IAA (28 femtomoles to 100 picomoles taken up over 60 minutes). The shape of the transport curve varies with the concentration of added IAA, although the rate of movement of the observed front of tracer is invariant with concentration. At the lowest concentration of tracer used, the labeled IAA in the transport stream is not detectably metabolized or immobilized, curvature does not develop as a result of tracer application, and normal phototropic and gravitropic responsiveness are not affected. Therefore we believe we are observing the transport of true tracer quantities of labeled auxin at this lowest concentration.

• OSTI ID: 5915108
• Journal Information: Plant Physiology; (USA), Vol. 94:2; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AUXINS
BIOCHEMICAL REACTION KINETICS
MAIZE
PLANT GROWTH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CEREALS
GRASS
GROWTH
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
PLANT GROWTH REGULATORS
PLANTS
REACTION KINETICS
551001* - Physiological Systems- Tracer Techniques