Two polypeptide chains in yeast transcription factor tau interact with DNA
Yeast transcription factor tau interacts with the A and B blocks of the intragenic promoter of tRNA genes. The structure of tau was investigated by identifying the polypeptide chains specifically complexed to the tRNA3Glu gene. Highly purified factor, obtained by an improved purification procedure, contained several polypeptide chains, four of which (Mr = 145,000, 135,000, 100,000 and 65,000) comigrated with tau-DNA complex by polyacrylamide gel electrophoresis. Antibodies raised against the 145- and 100-kDa components altered the migration of tau-DNA complexes in band shift assays and inhibited tRNA synthesis in a reconstituted transcription system. These components are immunologically unrelated proteins. By UV cross-linking to /sup 32/P-body-labeled tDNA followed by extensive DNase treatment, two polypeptides of the same size (145 and 100 kDa) were found to be radioactively labeled. Factor tau, therefore, appears to be a multisubunit DNA-binding protein with two distinct polypeptides contributing to DNA recognition. Limited proteolysis of tau generated a protease-resistant tau B (tau B) domain that binds solely to the B block. tau B-tDNA complexes were recognized by anti-145 IgG and contained a 120-kDa polypeptide that could originate from the 145-kDa component by proteolysis. These results strongly suggest that the 145-kDa polypeptide belongs to tau B and is responsible for B block binding.
- Research Organization:
- Centre d'Etudes Nucleaires de Saclay, Gif-sur-Yvette (France)
- OSTI ID:
- 5905894
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 264:13
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis and secretion of platelet-derived growth factor by human breast cancer cell lines
Glycoprotein Ib and glycoprotein IX in human platelets are acylated with palmitic acid through thioester linkages
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOCHEMICAL REACTION KINETICS
POLYPEPTIDES
TRANSFER RNA
GENES
ANTIGEN-ANTIBODY REACTIONS
CHROMATOGRAPHY
CROSS-LINKING
DNA-ASE
ELECTROPHORESIS
IN VITRO
MOLECULAR WEIGHT
PHOSPHORUS 32
SACCHAROMYCES CEREVISIAE
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRANSCRIPTION
ULTRAVIOLET RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
EUMYCOTA
FUNGI
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PLANTS
POLYMERIZATION
PROTEINS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RNA
SACCHAROMYCES
SEPARATION PROCESSES
YEASTS
550201* - Biochemistry- Tracer Techniques
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture