Detection of three protein binding sites in the serum-regulated promoter of the human gene encoding the 70 kDa heat shock protein

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.8.2203· OSTI ID:5903682

Wu, B J; Williams, G T; Morimoto, R I

The basal promoter of the human gene encoding 70-kDa heat shock protein (HSP70) controls maximal transcriptional activity and serum-regulated expression. The authors demonstrate that the three promoter elements defined by in vivo studies - CCAAT, serum-regulated element (SRE), and TATA - correspond to protein-binding sites in vitro. The promoter interactions with protein factors in HeLa cell crude nuclear extracts were detected by an exonuclease III digestion assay. The sequence specificity was demonstrated with promoter probes containing wild-type sequences or unique linker-scanner mutations that alter each of the elements. They suggest that the protein factor binding to the SRE is involved in the serum-regulated expression of the human gene for HSP70.

• Research Organization: Northwestern Univ., Evanston, IL (United States)
• OSTI ID: 5903682
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REPRESSORS
DNA SEQUENCING
PROTEINS
GENE REGULATION
ATP
BLOOD SERUM
HELA CELLS
IN VITRO
IN VIVO
MAN
PHOSPHORUS 32
TRANSCRIPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques