Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides
Abstract
The reaction of Octopus vulgaris hemocyanin with nitrite was studied under a variety of conditions in which the green half-met derivative is formed. Analytical evidence shows that the amount of chemically detectable nitrite in various samples of the derivative is not proportional to the cupric copper detected by EPR. The kinetics of oxidation of hemocyanin as a function of protein concentration and pH, in the presence of nitrite and ascorbate, is consistent with a scheme in which NO/sub 2/ is the reactive oxidant. We suggest that the green half-methemocyanin contains a metal center with one cuprous and one cupric copper without an exogenous nitrogen oxide ligand.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Padova (Italy)
- OSTI Identifier:
- 5900582
- Resource Type:
- Journal Article
- Journal Name:
- Biochemistry; (United States)
- Additional Journal Information:
- Journal Volume: 28:2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HEMOCYANIN; METABOLISM; NITRITES; BIOLOGICAL EFFECTS; NITROGEN OXIDES; BIOCHEMICAL REACTION KINETICS; ELECTRON SPIN RESONANCE; LIGANDS; MOLLUSCS; SPECTROPHOTOMETRY; ANIMALS; AQUATIC ORGANISMS; CHALCOGENIDES; INVERTEBRATES; KINETICS; MAGNETIC RESONANCE; METALLOPROTEINS; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PROTEINS; REACTION KINETICS; RESONANCE; 560300* - Chemicals Metabolism & Toxicology
Citation Formats
Salvato, B, Giacometti, G M, Beltramini, M, Zilio, F, Giacometti, G, Magliozzo, R S, and Peisach, J. Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides. United States: N. p., 1989.
Web. doi:10.1021/bi00428a040.
Salvato, B, Giacometti, G M, Beltramini, M, Zilio, F, Giacometti, G, Magliozzo, R S, & Peisach, J. Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides. United States. https://doi.org/10.1021/bi00428a040
Salvato, B, Giacometti, G M, Beltramini, M, Zilio, F, Giacometti, G, Magliozzo, R S, and Peisach, J. 1989.
"Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides". United States. https://doi.org/10.1021/bi00428a040.
@article{osti_5900582,
title = {Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides},
author = {Salvato, B and Giacometti, G M and Beltramini, M and Zilio, F and Giacometti, G and Magliozzo, R S and Peisach, J},
abstractNote = {The reaction of Octopus vulgaris hemocyanin with nitrite was studied under a variety of conditions in which the green half-met derivative is formed. Analytical evidence shows that the amount of chemically detectable nitrite in various samples of the derivative is not proportional to the cupric copper detected by EPR. The kinetics of oxidation of hemocyanin as a function of protein concentration and pH, in the presence of nitrite and ascorbate, is consistent with a scheme in which NO/sub 2/ is the reactive oxidant. We suggest that the green half-methemocyanin contains a metal center with one cuprous and one cupric copper without an exogenous nitrogen oxide ligand.},
doi = {10.1021/bi00428a040},
url = {https://www.osti.gov/biblio/5900582},
journal = {Biochemistry; (United States)},
number = ,
volume = 28:2,
place = {United States},
year = {Tue Jan 24 00:00:00 EST 1989},
month = {Tue Jan 24 00:00:00 EST 1989}
}
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