Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells

Beskrovnaya, O Yu; Fonshtein, M Yu; Kolibaba, L G; Yankovskii, N K; Debabov, V G

Title: Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells

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Abstract

Molecular cloning of Corynebacterium glutamicum genes for threonine and lysine synthesis has been done in Escherichia coli cells. The clonal library of EcoRI fragments of chromosomal DNA of C. glutamicum was constructed on the plasmid vector /lambda/pSL5. The genes for threonine and lysine synthesis were identified by complementation of E. coli mutations in thrB and lysA genes, respectively. Recombinant plasmids, isolated from independent ThrB/sup +/ clone have a common 4.1-kb long EcoRI DNA fragment. Hybrid plasmids isolated from LysA/sup +/ transductants of E. coli have common 2.2 and 3.3 kb long EcoRI fragments of C. glutamicum DNA. The hybrid plasmids consistently transduced the markers thrB/sup +/ and lysA/sup +/. The Southern hybridization analysis showed that the cloned DNA fragments hybridized with the fragments of identical length in C. glutamicum chromosomes.

Authors:: Beskrovnaya, O Yu; Fonshtein, M Yu; Kolibaba, L G; Yankovskii, N K; Debabov, V G

Publication Date:: Sun Jan 01 00:00:00 EST 1989

Research Org.:: All-Union Research Institute of Genetics and Breeding of Industrial Microorganisms, Moscow (USSR)

OSTI Identifier:: 5896388

Resource Type:: Journal Article

Journal Name:: Sov. Genet. (Engl. Transl.); (United States)

Additional Journal Information:: Journal Volume: 24:7; Other Information: Translated from Genetika; 24: No. 7, 1153-1158(Jul 1988)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ENDONUCLEASES; DNA-CLONING; LIGASES; LYSINE; BIOSYNTHESIS; PLASMIDS; THREONINE; BACTERIA; BACTERIOPHAGES; CHROMOSOMES; CLONE CELLS; DNA SEQUENCING; ELECTROPHORESIS; ESCHERICHIA COLI; GENE MUTATIONS; HYBRIDIZATION; LABELLED COMPOUNDS; MOLECULAR STRUCTURE; PHOSPHORUS 32; RADIOASSAY; TRANSCRIPTION; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CELL CULTURES; CLONING; DAYS LIVING RADIOISOTOPES; DNA HYBRIDIZATION; DNA-ASE; ENZYMES; ESTERASES; HYDROLASES; HYDROXY ACIDS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; MUTATIONS; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PARASITES; PHOSPHODIESTERASES; PHOSPHORUS ISOTOPES; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; SYNTHESIS; VIRUSES; 550401* - Genetics- Tracer Techniques; 550701 - Microbiology- Tracer Techniques; 550301 - Cytology- Tracer Techniques

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                    Beskrovnaya, O Yu, Fonshtein, M Yu, Kolibaba, L G, Yankovskii, N K, and Debabov, V G. Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells.  United States: N. p., 1989. 
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                    Beskrovnaya, O Yu, Fonshtein, M Yu, Kolibaba, L G, Yankovskii, N K, & Debabov, V G. Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells.  United States.

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                    Beskrovnaya, O Yu, Fonshtein, M Yu, Kolibaba, L G, Yankovskii, N K, and Debabov, V G. 1989.  
        "Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells".  United States.

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@article{osti_5896388,

  title        = {Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells},

  author       = {Beskrovnaya, O Yu and Fonshtein, M Yu and Kolibaba, L G and Yankovskii, N K and Debabov, V G},

  abstractNote = {Molecular cloning of Corynebacterium glutamicum genes for threonine and lysine synthesis has been done in Escherichia coli cells. The clonal library of EcoRI fragments of chromosomal DNA of C. glutamicum was constructed on the plasmid vector /lambda/pSL5. The genes for threonine and lysine synthesis were identified by complementation of E. coli mutations in thrB and lysA genes, respectively. Recombinant plasmids, isolated from independent ThrB/sup +/ clone have a common 4.1-kb long EcoRI DNA fragment. Hybrid plasmids isolated from LysA/sup +/ transductants of E. coli have common 2.2 and 3.3 kb long EcoRI fragments of C. glutamicum DNA. The hybrid plasmids consistently transduced the markers thrB/sup +/ and lysA/sup +/. The Southern hybridization analysis showed that the cloned DNA fragments hybridized with the fragments of identical length in C. glutamicum chromosomes.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5896388},
  journal      = {Sov. Genet. (Engl. Transl.); (United States)},
number       = ,

  volume       = 24:7,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 1989},

  month        = {Sun Jan 01 00:00:00 EST 1989}

}

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