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Title: Antigen-binding site protection during radiolabeling leads to a higher immunoreactive fraction

Abstract

It is generally accepted that the immunointegrity of an antibody (Ab) depends on the preservation of its antigen-binding sites. Our goal was to radiolabel an antibody at several iodine:antibody molar ratios under conditions protecting its combining site and to compare its immunoreactive fraction (IRF) and electrophoretic mobility with those of the same antibody radiolabeled without protection. The data indicate that an antibody radiolabeled while its antigen-binding site is occupied by its antigen had the same IRF, regardless of the number of iodine atoms per antibody molecule. On the other hand, even at an I:Ab ratio of 1:1, the IRF of the same antibody radiolabeled without protection was lower than that of a protected one and decreased with increasing I:Ab ratios. In addition, the iodination of these Ab changes their electrophoretic mobility; however, when the Ab is labeled in the protected state, the degree of change is less. The binding of an antibody to its antigen prior to radiolabeling, therefore, enhances its immuno-integrity and prevents major conformational changes as reflected by electrophoresis.

Authors:
; ; ; ; ;  [1]
  1. Harvard Medical School, Boston, MA (USA)
Publication Date:
OSTI Identifier:
5895690
Resource Type:
Journal Article
Journal Name:
Journal of Nuclear Medicine; (USA)
Additional Journal Information:
Journal Volume: 32:1; Journal ID: ISSN 0161-5505
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIGENS; RECEPTORS; MONOCLONAL ANTIBODIES; LABELLING; ANTIGEN-ANTIBODY REACTIONS; ELECTROPHORESIS; GOATS; IMMUNE REACTIONS; IODINE ISOTOPES; RABBITS; TRACER TECHNIQUES; ANIMALS; ANTIBODIES; DOMESTIC ANIMALS; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; PROTEINS; RUMINANTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Van den Abbeele, A D, Aaronson, R A, Daher, S, Taube, R A, Adelstein, S J, and Kassis, A I. Antigen-binding site protection during radiolabeling leads to a higher immunoreactive fraction. United States: N. p., 1991. Web.
Van den Abbeele, A D, Aaronson, R A, Daher, S, Taube, R A, Adelstein, S J, & Kassis, A I. Antigen-binding site protection during radiolabeling leads to a higher immunoreactive fraction. United States.
Van den Abbeele, A D, Aaronson, R A, Daher, S, Taube, R A, Adelstein, S J, and Kassis, A I. 1991. "Antigen-binding site protection during radiolabeling leads to a higher immunoreactive fraction". United States.
@article{osti_5895690,
title = {Antigen-binding site protection during radiolabeling leads to a higher immunoreactive fraction},
author = {Van den Abbeele, A D and Aaronson, R A and Daher, S and Taube, R A and Adelstein, S J and Kassis, A I},
abstractNote = {It is generally accepted that the immunointegrity of an antibody (Ab) depends on the preservation of its antigen-binding sites. Our goal was to radiolabel an antibody at several iodine:antibody molar ratios under conditions protecting its combining site and to compare its immunoreactive fraction (IRF) and electrophoretic mobility with those of the same antibody radiolabeled without protection. The data indicate that an antibody radiolabeled while its antigen-binding site is occupied by its antigen had the same IRF, regardless of the number of iodine atoms per antibody molecule. On the other hand, even at an I:Ab ratio of 1:1, the IRF of the same antibody radiolabeled without protection was lower than that of a protected one and decreased with increasing I:Ab ratios. In addition, the iodination of these Ab changes their electrophoretic mobility; however, when the Ab is labeled in the protected state, the degree of change is less. The binding of an antibody to its antigen prior to radiolabeling, therefore, enhances its immuno-integrity and prevents major conformational changes as reflected by electrophoresis.},
doi = {},
url = {https://www.osti.gov/biblio/5895690}, journal = {Journal of Nuclear Medicine; (USA)},
issn = {0161-5505},
number = ,
volume = 32:1,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}