Modulation of lymphopoiesis
Ongoing experiments contribute to the four specific aims in our current 3-year project period studying lymphopoiesis using CE mammary adenocarcinoma (CE maca) mice. Specific Aim 1: Function of Bone Marrow Stroma in CE Maca Bearing Mice. We have concluded that bone marrow stromal cells from tumor bearing animals are as effective as bone marrow stromal cells from normal animals at supporting B lymphopoiesis in culture. If stromal cell disfunction occurs in tumor bearing animals, the effect is not long lasting. Specific Aim 2: Factors Elaborated by CE Maca. Conditioned medium from CE maca cultures induces bone resorbing activity in bone organ cultures. We have identified a 32 kDa protein that meets the criteria for an osteoclast specific growth factor in this medium. However, we have also found that even though an osteoclast specific growth factor exists, a growth factor whose primary targets are granulocyte precursors will also stimulate osteoclast activity. Specific Aim 3: In Vitro Systems for Investigating Modulation of NK Lymphopoiesis by CE Maca. CE maca is shown to inhibit NK all production as well as B all production. In the course of the these experiments, we have established conditions for enriching NK precursors and have established a prototype long term bone marrow culture system which shows NK cell production. Specific Aim 4: Relationship Between Myclogenic and Intrathymic T Cell Precursors. There is evidence for a stream of T all maturation in both host (or intrathymic) cells and non-host (both hematogenous and intrathymic) cells. If thymic atrophy is induced by cortisone (a depopulation equivalent to that caused by 1000 rad) our data conclusively show thymic recovery is effected almost exclusively by intrathymic cells. This assigns a much greater importance to intrathymic T all progenitors in the maintenance of T cell production in the thymus than has been hitherto recognized. (MHB)
- Research Organization:
- Washington Univ., Seattle, WA (USA). Dept. of Biological Structure
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG06-86ER60409
- OSTI ID:
- 5892358
- Report Number(s):
- DOE/ER/60409-5; ON: DE91009592
- Country of Publication:
- United States
- Language:
- English
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Modulation of lymphopoiesis. Comprehensive progress report, January 1, 1991--July 30, 1991
Modulation of lymphopoiesis
Related Subjects
BONE MARROW
LEUKOPOIESIS
SPLEEN
THYMUS
BIOLOGICAL REGENERATION
ANTIGENS
ATROPHY
BIOLOGICAL PATHWAYS
BONE MARROW CELLS
BONE TISSUES
CARCINOMAS
CHROMIUM 51
CORTISONE
CULTURE MEDIA
DIFFUSION CHAMBERS
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MICE
PROGRESS REPORT
RADIATION EQUIVALENCE
STEM CELLS
THYMUS CELLS
TISSUE CULTURES
ADRENAL HORMONES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DISEASES
DOCUMENT TYPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOCORTICOIDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KETONES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MITOGENS
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PREGNANES
PROTEINS
RADIOISOTOPES
RECOVERY
RODENTS
SOMATIC CELLS
STEROIDS
TISSUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550900 - Pathology
550300 - Cytology