Human cytoplasmic actin proteins are encoded by a multigene family

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.6.674· OSTI ID:5891850

Engel, J; Gunning, P; Kedes, L

The authors characterized nine human actin genes that they isolated from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and ..cap alpha..-, ..beta..-, and ..gamma..-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria they show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5' and 3' ends of the coding region of a cloned chicken ..beta..-actin cDNA. They conclude, therefore, that the human cytoplasmic actin proteins are encoded by a multigene family.

• Research Organization: Howard Hughes Medical Institute and Dept. of Medicine, Stanford Univ. School of Medicine and Veterans Administration Medical Center, Palo Alto, CA 94305
• OSTI ID: 5891850
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 2:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACTIN
DNA-CLONING
GENES
GENETIC MAPPING
GENETIC VARIABILITY
CHICKENS
ENZYME INHIBITORS
GENETIC CONTROL
HYBRIDIZATION
MAN
MESSENGER-RNA
MUSCLES
TEMPERATURE EFFECTS
TRANSCRIPTION
ANIMALS
BIOLOGICAL VARIABILITY
BIRDS
CLONING
CONTROL
FOWL
MAMMALS
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEST CONTROL
PRIMATES
PROTEINS
RNA
VERTEBRATES
550200* - Biochemistry