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Title: Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors

Abstract

A series of deletions and insertions utilizing the herpesvirus thymidine kinase gene (tk) were constructed in the murine retrovirus Friend spleen focus-forming virus (SFFV). In all cases, the coding region for the SFFV-specific glycoprotein (gp55), which is implicated in erythroleukemic transformation, was left intact. These SFFV-TK and SFFV deletion vectors were analyzed for expression of tk and gp55 after DNA-mediated gene transfer. In addition, virus rescued by cotranfection of these vectors with Moloney murine leukemia virus was analyzed for infectious TK-transducing virus, gp55 expression, and erythroleukemia-inducing ability. The experiments demonstrated that deletions or insertions within the intron for the gp55 env gene can interfere with expression of gp55 after both DNA-mediated gene transfer and virus infection. In contrast, the gene transfer efficiency of the tk gene was unaffected in the SFFV-TK vectors, and high-titer infectious TK virus could be recovered. Revertant viruses capable of inducing erythroleukemia and expressing gp55 were generated after cotranfection of the SFFV-TK vectors with murine leukemia virus. The revertant viruses lost both tk sequences and the ability to transduce TK/sup -/ fibroblasts to a TK/sup +/ phenotype. These experiments demonstrate that segregation of the TK and erythroleukemia functions can occur in retrovirus vectors which initially carrymore » both markers.« less

Authors:
;
Publication Date:
Research Org.:
Ontario Cancer Institute and Dept. of Medical Biophysics, Univ. of Toronto, Toronto, Ontario M4X 1K9
OSTI Identifier:
5891557
Resource Type:
Journal Article
Journal Name:
Mol. Cell. Biol.; (United States)
Additional Journal Information:
Journal Volume: 3:12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HERPES SIMPLEX; MUTAGENESIS; LEUKEMIA VIRUSES; REVERTANTS; ONCOGENES; DNA SEQUENCING; PHOSPHOTRANSFERASES; GENES; GENETIC ENGINEERING; CHROMOSOME LOSSES; ERYTHROCYTES; FIBROBLASTS; GLUCOPROTEINS; ONCOGENIC TRANSFORMATIONS; PHENOTYPE; RECOMBINANT DNA; RNA PROCESSING; SPLEEN; STRUCTURAL MODELS; THYMIDINE; VIRUSES; ANIMAL CELLS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOHYDRATES; CELL TRANSFORMATIONS; CONNECTIVE TISSUE CELLS; DISEASES; DNA; ENZYMES; HETEROCYCLIC COMPOUNDS; INFECTIOUS DISEASES; LOSSES; MATERIALS; MICROORGANISMS; MUTANTS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PARASITES; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; PYRIMIDINES; RIBOSIDES; SACCHARIDES; SKIN DISEASES; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; VIRAL DISEASES; 550400* - Genetics

Citation Formats

Joyner, A L, and Bernstein, A. Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors. United States: N. p., 1983. Web. doi:10.1128/MCB.3.12.2191.
Joyner, A L, & Bernstein, A. Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors. United States. https://doi.org/10.1128/MCB.3.12.2191
Joyner, A L, and Bernstein, A. 1983. "Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors". United States. https://doi.org/10.1128/MCB.3.12.2191.
@article{osti_5891557,
title = {Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors},
author = {Joyner, A L and Bernstein, A},
abstractNote = {A series of deletions and insertions utilizing the herpesvirus thymidine kinase gene (tk) were constructed in the murine retrovirus Friend spleen focus-forming virus (SFFV). In all cases, the coding region for the SFFV-specific glycoprotein (gp55), which is implicated in erythroleukemic transformation, was left intact. These SFFV-TK and SFFV deletion vectors were analyzed for expression of tk and gp55 after DNA-mediated gene transfer. In addition, virus rescued by cotranfection of these vectors with Moloney murine leukemia virus was analyzed for infectious TK-transducing virus, gp55 expression, and erythroleukemia-inducing ability. The experiments demonstrated that deletions or insertions within the intron for the gp55 env gene can interfere with expression of gp55 after both DNA-mediated gene transfer and virus infection. In contrast, the gene transfer efficiency of the tk gene was unaffected in the SFFV-TK vectors, and high-titer infectious TK virus could be recovered. Revertant viruses capable of inducing erythroleukemia and expressing gp55 were generated after cotranfection of the SFFV-TK vectors with murine leukemia virus. The revertant viruses lost both tk sequences and the ability to transduce TK/sup -/ fibroblasts to a TK/sup +/ phenotype. These experiments demonstrate that segregation of the TK and erythroleukemia functions can occur in retrovirus vectors which initially carry both markers.},
doi = {10.1128/MCB.3.12.2191},
url = {https://www.osti.gov/biblio/5891557}, journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 3:12,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 1983},
month = {Thu Dec 01 00:00:00 EST 1983}
}