Gene order for rubella virus structural proteins is NH/sub 2/-C-E2-E1-COOH

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · J. Virol.; (United States)

OSTI ID:5889425

Oker-Blom, C

The order of translation in vivo of the genes coding for rubella virus structural proteins was studied in infected B-Vero cells. The proteins were sequentially pulse-chase labeled with (/sup 35/S)methionine after synchronization of translation initiation with hypertonic salt treatment. A sequential labeling procedure (window-labeling) to specifically label defined segments of the structural proteins was also used. The labeled proteins were identified by sodium dodecyl sulfate-gel electrophoresis after immunoprecipitation with specific antisera directed against the two virion glycoproteins (E1 and E2a/E2b) and the nucleocapsid (C) protein. The order of translation was found to be NH/sub 2/-C-E2-E1-COOH. We have previously shown that the structural proteins are synthesized in vitro from a cytoplasmic 24S subgenomic mRNA as a 110,000-dalton (p110) precursor. Here, it is shown that p110 is precipitated with anti-C, anti-E2, and anti-E1 sera, indicating that p110 is the precursor of all three structural proteins. Two major in vitro translation products (M/sub r/s, 66,000 and 62,000) that could represent preterminated polypeptide chains or proteolytic cleavage products were precipitated with anti-C and anti-Es sera, but not with anti-E1 serum, indicating, in conformity with the in vivo results, that the genes for the C and E2 proteins are adjacent to each other. Using these specific antisera, we have also confirmed the identity of the unglycosylated forms of E1 (M/sub r/, 53,000) and E2 (M/sub r/ 30,000) immunoprecipitated from tunicamycin-treated infected cells. 18 references, 6 figures.

• Research Organization: Univ. of Helsinki, Finland
• OSTI ID: 5889425
• Journal Information: J. Virol.; (United States), Vol. 51:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MEASLES VIRUS
GENETIC MAPPING
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
EXPERIMENTAL DATA
IMMUNE REACTIONS
IN VIVO
LABELLED COMPOUNDS
METHIONINE
PRECURSOR
RECOMBINANT DNA
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DATA
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
EVEN-ODD NUCLEI
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAPPING
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
RADIOISOTOPES
SULFUR ISOTOPES
VIRUSES
550401* - Genetics- Tracer Techniques