Visualization of Trichoderma reesei cellobiohydrolase I and endoglucanase I on aspen cellulose by using monoclonal antibody-colloidal gold conjugates

Journal Article · Fri Nov 01 00:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5883818

Nieves, R A; Grohmann, K; Himmel, M E ^[1]; Ellis, R P; Todd, R J; Johnson, T J.A. ^[2]

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1. Solar Energy Research Inst., Golden, CO (United States)
2. Colorado State Univ., Fort Collins, Co (United States)

Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immobilization of cellulose microfibrils on copper electron microscopy grids, incubation of the cellulose-coated grids with cellulase(s), binding of MAb-colloidal gold conjugates to cellulase(s), and visualization via transmission electron microscopy. CBH I was seen bound to apparent crystalline cellulose as well as apparent amorphous cellulose. EG I was seen bound extensively to apparent amorphous cellulose with minimal binding to crystalline cellulose.

• OSTI ID: 5883818
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:11; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELLULOSE
BIODEGRADATION
FUNGI
ENZYME ACTIVITY
CELLULASE
MONOCLONAL ANTIBODIES
TRANSMISSION ELECTRON MICROSCOPY
ANTIBODIES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ELECTRON MICROSCOPY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MICROSCOPY
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
550200* - Biochemistry