Counterstaining human chromosomes for flow karyology

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990050605· OSTI ID:5881833

Meyne, J; Bartholdi, M F; Travis, G; Cram, L S

Isolated human metaphase chromosomes stained with the fluorochromes 4'-6-diamino-2-phenylindole (DAPI) and chromomycin A/sub 3/ (CA/sub 3/), and counterstained with nonfluorescent netropsin (NTR), have been analyzed by dual-laser flow cytometry. Counterstaining with NTR reduced DAPI fluorescence except at regions on chromosomes 1,9,15,16, and Y, corresponding to C-band heterochromatin. Bivariate flow karyology of human chromosomes treated with this triple-stain combination resolves chromosomes 1,9, and Y distinctly from the remaining chromosomes and resolves variations between chromosome homologues not detected by staining with propidium iodide (PI) or with the double stain combination Hoechst 33258 (HO) and CA/sub 3/. 15 references, 5 figures.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5881833
• Journal Information: Cytometry (Baltimore); (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
CYTOLOGICAL TECHNIQUES
CELL FLOW SYSTEMS
FLUORESCENCE
HETEROCHROMATIN
SAMPLE PREPARATION
STAINS
CHROMATIN
LUMINESCENCE
550300* - Cytology