Transfected DNA is mutated in monkey, mouse, and human cells

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.10.1951· OSTI ID:5877904

Lebkowski, A S; Du Bridge, R B; Antell, E A; Greisen, K S; Calos, M P

Papovavirus-based shuttle vectors containing the bacterial lacI gene were used to show that a mutation frequency in the range of 1% occurs in lacI when such vectors are transfected into COS7 and CV-1 simian cells, NIH 3T3, 3T6, L, and C127 mouse cells, and human 293 and HeLa cells. This frequency is approximately four orders of magnitude higher than the spontaneous mutation frequency in either mammalian or bacterial cells. The mutations are predominantly base substitutions and deletions and also include insertions from the mammalian genome. Time course experiments argue that mutagenesis occurs soon after arrival of the DNA into the nucleus. However, replication of the vector is not required since mutations occur even when the vector lacks all viral sequences. The high mutation frequency appears to be the characteristic outcome of transfection of DNA into mammalian cells.

• Research Organization: Dept. of Genetics, Stanford Univ. School of Medicine, Stanford, CA 94305
• OSTI ID: 5877904
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 4:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
DNA SEQUENCING
GENETIC MAPPING
BACTERIOPHAGES
DNA REPLICATION
HELA CELLS
SIMIAN VIRUS
VIRULENCE
CHROMOSOME LOSSES
DEMONSTRATION PROGRAMS
GENETIC ENGINEERING
GENOME MUTATIONS
HUMAN POPULATIONS
MONKEYS
MUTAGENESIS
MUTATION FREQUENCY
RATS
ANIMALS
LOSSES
MAMMALS
MAPPING
MICROORGANISMS
MUTATIONS
NUCLEIC ACID REPLICATION
PARASITES
POPULATIONS
PRIMATES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550400* - Genetics