Effects of age on muscarinic agonist-induced contraction an IP accumulation in airway smooth muscle

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90305-U· OSTI ID:5865521

Wills-Karp, M ^[1]

---

1. Johns Hopkins Univ., Baltimore, MD (United States)

The effects of age on carbachol-stimulated force development and ({sup 3}H)inositol phosphate production was studied in tracheal rings from guinea pigs aged 1 month and 25 months of age. The pD{sub 2} for the contractile response to carbachol was significantly reduced in tracheal tissues from old animals as compared to that of the young tissues, respectively. In contrast, inositol phosphate formation was not altered with increasing age when stimulated by carbachol or NaF, a direct activator of G proteins. Carbachol-induced inositol phosphate accumulation was inhibited by treatment with 1{mu}g/ml pertussis toxin, suggesting that IP1 accumulation is coupled to a pertussis-toxin-sensitive protein. The pD{sub 2} values for contraction were significantly different from the pD{sub 2} values for IP1 accumulation, in both young and old tissues, respectively. These data suggest that IP1 accumulation is not responsible for the decreased contractile ability in tracheal smooth muscle during aging.

• OSTI ID: 5865521
• Journal Information: Life Sciences; (United States), Vol. 49:14; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
CONTRACTION
PHOSPHOLIPIDS
BIOSYNTHESIS
SYMPATHOMIMETICS
BIOCHEMICAL REACTION KINETICS
AGE DEPENDENCE
GUINEA PIGS
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
DRUGS
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques