Human liver estrone (E1), Estradiol (E2) and dehydroepiandrosterone (DHEA) sulfotransferases (STs): Comparison with thermostable (TS) and thermolabile (TL) phenol sulfotransferase (PST) activities
- Mayo Clinic/Mayo Foundation, Rochester, MN (United States)
Sulfation plays an important role in the metabolism of E1, E2 and DHEA in humans. The relationship between the enzymes that catalyze the sulfation of E1, E2 and DHEA and TS and TL PST is unclear. The authors compared thermal stability, sensitivity to inhibition by 2,6-dichloro-4-nitrophenol (DCNP) and individual variation in the regulation of these steroid ST activities with those of TS PST and TL PST in the human liver. E2 ST and TS PST had very similar thermal stabilities. The thermal inactivation profile of E1 ST suggested that this activity might be related to both DHEA ST and TS PST. DCNP inhibition studies also showed similar profiles for E2 ST and TS PST, with a small resistant component for E2 ST. A multiphasic profile for DCNP inhibition of E1 ST activity was found. Finally, studies performed with human liver sample showed significant correlations between E2 ST and TS PST, E1 ST and DHEA ST, E2 St and E1 ST, and, to a lesser degree, between E1 ST and TS PST and E2 ST and DHEA ST. TL PST was not correlated significantly with any of the other activities. These results suggest that the sulfation of E2 in human liver is catalyzed predominantly by TS PST, although DHEA ST may also play a role. Their results also suggest that the sulfation of E1 is catalyzed by DHEA ST and by TS PST, although other ST(s) could also be involved.
- OSTI ID:
- 5863594
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Thermolabile phenol sulfotransferase gene (STM): Localization to human chromosome 16p11.2
Human phenol sulfotransferase STP2 gene: Molecular cloning, structural characterization, and chromosomal localization
Related Subjects
ANDROSTERONE
METABOLISM
ESTRADIOL
ESTRONE
NITRO COMPOUNDS
BIOLOGICAL EFFECTS
TRANSFERASES
ENZYME ACTIVITY
INHIBITION
LIVER
MAN
STABILITY
ANDROGENS
ANDROSTANES
ANIMALS
BODY
DIGESTIVE SYSTEM
ENZYMES
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PROTEINS
STEROID HORMONES
STEROIDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology