Purified rat liver DT-diaphorase potentiates the mutagenicity of 1,3-, 1,6- and 1,8-dinitropyrene

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5863333

Hajos, A K.D.; Winston, G W ^[1]

---

1. Louisiana State Univ., Baton Rouge (United States)

The effect of highly purified rat liver cytosolic DT-diaphorase on the mutagenicity of 1,3-, 1,6- and 1,8-dinitropyrene (DNP) was studied in the Ames Salmonella typhimurium mutagenicity assay. DT-diaphorase over the range of 0 - 0.78 {mu}g/plate increased the mutagenicity of all three DNP in S. typhimurium TA 98 to a maximum of 3-fold. In the nitroreductase-deficient strain, TA98NR, 1,6- and 1,8-DNP were activated to a lesser extent in the presence of equal amounts of DT-diaphorase as compared to TA98. The mutagenicity of 1,3-DNP was markedly lowered in TA98NR and DT-diaphorase did not increase the mutagenicity of this toxicant except at the highest level utilized. DT-diaphorase was found to activate 1,6-, but not 1,3-DNP to mutagenic intermediates in TA98 18DNP{sub 6}, a strain deficient in O-acetyltransferase activity. The results suggest that DT-diaphorase not only catalyzes reduction of the parent DNP but also that of partially reduced metabolites generated from that DNP. This may result in increased formation of the penultimate mutagenic species. Thus, while DT-diaphorase is commonly viewed as having antimutagenic properties with certain compounds the opposite appears to be true for DNP.

• OSTI ID: 5863333
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NITRO COMPOUNDS
MUTAGEN SCREENING
OXIDOREDUCTASES
ENZYME ACTIVITY
LIVER
METABOLIC ACTIVATION
RATS
SALMONELLA TYPHIMURIUM
ANIMALS
BACTERIA
BODY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
MAMMALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
RODENTS
SALMONELLA
SCREENING
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology