Regulation and localization of amylolytic enzymes in Clostridium acetobutylicum ATCC 824

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:5859781

Annous, B A; Blaschek, H P ^[1]

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1. Univ. of Illinois, Urbana (USA)

Amylolytic activity was primarily cell associated when Clostridium acetobutylicum was grown on glucose or maltose and primarily extracellular when grown on dextrin or starch. Total amylolytic activity decreased with increasing glucose concentration. When this microorganism was grown in P2 medium containing starch, the intracellular amylolytic activity was 90% membrane bound and 10% cytoplasmic in nature. The addition of 1% glucose to 2% starch-based P2 medium at different stages of growth indicated that this carbohydrate repressed the synthesis of C. acetobutylicum amylolytic enzymes at the level of transcription.

• OSTI ID: 5859781
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:8; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CLOSTRIDIUM ACETOBUTYLICUM
ENZYME ACTIVITY
GLUCOSE
INHIBITION
STARCH
TRANSCRIPTION
ALDEHYDES
BACTERIA
CARBOHYDRATES
CLOSTRIDIUM
HEXOSES
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
REAGENTS
SACCHARIDES
550200* - Biochemistry