Sensitive radioenzymatic assay for catechol drugs

Journal Article · Tue Jan 01 00:00:00 EST 1980 · J. Neurosci. Res.; (United States)

DOI:https://doi.org/10.1002/jnr.490050613· OSTI ID:5858683

Durrett, L R; Ziegler, M G

This assay measures picogram quantities of catechol drugs and endogenous catecholamines in body tissues and fluids. The catechols are converted to their 3H-O-methyl metabolites during incubation with 3H-S-adenosylmethionine then separated by solvent extraction and thin-layer chromatography. Most drugs containing the catechol structure can be radiolabeled and separated from norepinephrine and epinephrine by this technique to provide simultaneous measurement of endogenous and exogenously administered catechols. The disposition of isoproterenol in tissues and fluids of man and experimental animals is measured to illustrate the utility of this assay. The reactivity of several commonly administered catechol drugs with COMT is described and the possible implications discussed.

• OSTI ID: 5858683
• Journal Information: J. Neurosci. Res.; (United States), Vol. 5:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CATECHOLAMINES
RADIOASSAY
CHEMICAL PREPARATION
PHARMACOLOGY
RADIOPHARMACEUTICALS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AROMATICS
DISTRIBUTION
DRUGS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques