Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · J. Nutr.; (United States)

OSTI ID:5856909

Kipp, D E; Rivers, J M

The effect of a single injection of ACTH (3 IU/100 g body weight) on the distribution of ascorbic acid (AA) and radiolabeled AA in 20 tissues was studied in adult male guinea pigs consuming 500 mg AA/kg diet. Saline- or ACTH-injected animals were simultaneously injected with (1-/sup 14/C)AA, and killed at 0.5, 1, 2, 4 and 6 h after injection. There was no significant difference between treatments in the weight of any tissue over the 6-h experimental period. As anticipated, the concentration of AA in the adrenals of animals injected with ACTH was 33% of that of animals injected with saline at 4 h. Unexpectedly, the concentration of radiolabeled AA in the adrenals at 0.5 h after ACTH injection was 172% of that after saline injection. The concentration of radiolabeled AA in the adrenal of the saline-injected animals increased slowly over time to reach a level similar to that of ACTH-injected animals by 6 h. There was no effect of ACTH on the level of AA or uptake in any of the other tissues examined. These results demonstrate that a single dose of ACTH markedly influences the retention of AA in the adrenal gland without similarly altering retention of AA in other tissues. Furthermore, ACTH treatment causes both accelerated uptake and release of AA into the adrenals.

• Research Organization: Cornell Univ., Ithaca, NY
• OSTI ID: 5856909
• Journal Information: J. Nutr.; (United States), Vol. 117:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCORBIC ACID
TISSUE DISTRIBUTION
HYDROCORTISONE
BIOLOGICAL FUNCTIONS
ADRENAL GLANDS
CARBON 14 COMPOUNDS
GUINEA PIGS
ADRENAL HORMONES
ANIMALS
BODY
CORTICOSTEROIDS
DISTRIBUTION
ENDOCRINE GLANDS
FUNCTIONS
GLANDS
GLUCOCORTICOIDS
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RODENTS
STEROIDS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques