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Title: Regulation of atrial natriuretic peptide receptors in the rat brain

Abstract

We have studied the localization, kinetics, and regulation of receptors for the circulating form of the atrial natriuretic peptide (ANP; 99-126) in the rat brain. Quantitative autoradiographic techniques and a /sup 125/I-labeled ligand, /sup 125/I-ANP (99-126), were employed. After in vitro autoradiography, quantification was achieved by computerized microdensitometry followed by comparison with /sup 125/I-standards. ANP receptors were discretely localized in the rat brain, with the highest concentrations in circumventricular organs, the choroid plexus, and selected hypothalamic nuclei involved in the production of the antidiuretic hormone vasopressin and in blood-pressure control. Spontaneously (genetic) hypertensive rats showed much lower numbers of ANP receptors than normotensive controls in the subfornical organ, the area postrema, the nucleus of the solitary tract, and the choroid plexus. These changes are in contrast to those observed for receptors of angiotensin II, another circulating peptide with actions opposite to those of ANP. Under conditions of acute dehydration after water deprivation, as well as under conditions of chronic dehydration such as those present in homozygous Brattleboro rats, there was an up-regulation of ANP receptors in the subfornical organ. Our results indicate that in the brain, circumventricular organs contain ANP receptors which could respond to variations in the concentration ofmore » circulating ANP. In addition, brain areas inside the blood-brain barrier contain ANP receptors probably related to the endogenous, central ANP system. The localization of ANP receptors and the alterations in their regulation present in genetically hypertensive rats and after dehydration indicate that brain ANP receptors are probably related to fluid regulation, including the secretion of vasopressin, and to cardiovascular function.« less

Authors:
Publication Date:
Research Org.:
National Institute of Mental Health, Bethesda, MD
OSTI Identifier:
5856771
Resource Type:
Journal Article
Journal Name:
Cell. Mol. Neurobiol.; (United States)
Additional Journal Information:
Journal Volume: 7:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; RECEPTORS; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL LOCALIZATION; AUTORADIOGRAPHY; BIOLOGICAL FUNCTIONS; BLOOD PRESSURE; BRAIN; HYPERTENSION; IODINE 125; RATS; TISSUE DISTRIBUTION; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR DISEASES; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DISEASES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; FUNCTIONS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SYMPTOMS; VASCULAR DISEASES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Saavedra, J M. Regulation of atrial natriuretic peptide receptors in the rat brain. United States: N. p., 1987. Web. doi:10.1007/BF00711552.
Saavedra, J M. Regulation of atrial natriuretic peptide receptors in the rat brain. United States. https://doi.org/10.1007/BF00711552
Saavedra, J M. 1987. "Regulation of atrial natriuretic peptide receptors in the rat brain". United States. https://doi.org/10.1007/BF00711552.
@article{osti_5856771,
title = {Regulation of atrial natriuretic peptide receptors in the rat brain},
author = {Saavedra, J M},
abstractNote = {We have studied the localization, kinetics, and regulation of receptors for the circulating form of the atrial natriuretic peptide (ANP; 99-126) in the rat brain. Quantitative autoradiographic techniques and a /sup 125/I-labeled ligand, /sup 125/I-ANP (99-126), were employed. After in vitro autoradiography, quantification was achieved by computerized microdensitometry followed by comparison with /sup 125/I-standards. ANP receptors were discretely localized in the rat brain, with the highest concentrations in circumventricular organs, the choroid plexus, and selected hypothalamic nuclei involved in the production of the antidiuretic hormone vasopressin and in blood-pressure control. Spontaneously (genetic) hypertensive rats showed much lower numbers of ANP receptors than normotensive controls in the subfornical organ, the area postrema, the nucleus of the solitary tract, and the choroid plexus. These changes are in contrast to those observed for receptors of angiotensin II, another circulating peptide with actions opposite to those of ANP. Under conditions of acute dehydration after water deprivation, as well as under conditions of chronic dehydration such as those present in homozygous Brattleboro rats, there was an up-regulation of ANP receptors in the subfornical organ. Our results indicate that in the brain, circumventricular organs contain ANP receptors which could respond to variations in the concentration of circulating ANP. In addition, brain areas inside the blood-brain barrier contain ANP receptors probably related to the endogenous, central ANP system. The localization of ANP receptors and the alterations in their regulation present in genetically hypertensive rats and after dehydration indicate that brain ANP receptors are probably related to fluid regulation, including the secretion of vasopressin, and to cardiovascular function.},
doi = {10.1007/BF00711552},
url = {https://www.osti.gov/biblio/5856771}, journal = {Cell. Mol. Neurobiol.; (United States)},
number = ,
volume = 7:2,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 1987},
month = {Mon Jun 01 00:00:00 EDT 1987}
}