Role of quinate dehydrogenase in quinic acid metabolism in conifers

Journal Article · Sun Aug 10 00:00:00 EDT 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5856485

Osipov, V I; Shein, I V

Quinate dehydrogenase was isolated from young needles of the Siberian larch and partially purified by ammonium sulfate fractionation. It was found that in conifers, in contrast to other plants, quinate dehydrogenase is active both with NAD and with NADP. The values of K/sub m/ for quinate and NADP were 1.8 and 0.18 mM. The enzyme exhibits maximum activity at pH 9.0. It was assumed that NADP-dependent quinate dehydrogenase is responsible for quinic acid synthesis. The special features of the organization and regulation of the initial stages of the shikimate pathway in conifers are discussed.

• Research Organization: V.N. Sukachev Institute of Lumber and Wood, Krasnoyarsk, USSR
• OSTI ID: 5856485
• Journal Information: Biochemistry (Engl. Transl.); (United States), Vol. 51:2; Other Information: Translated from Biokhimiya; 51: No. 2, 230-236(Feb 1986)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CONIFERS
BIOCHEMISTRY
ORGANIC ACIDS
METABOLISM
RADIOENZYMATIC ASSAY
OXIDOREDUCTASES
ENZYME ACTIVITY
QUINONES
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
FRACTIONATION
LABELLING
NAD
NADP
PH VALUE
RADIOACTIVITY
SHIKIMIC ACID
AROMATICS
CARBOXYLIC ACIDS
CHEMISTRY
COENZYMES
ENZYMES
HYDROXY ACIDS
LABELLED COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PLANTS
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques