Influence of natural and recombinant interferons on development of antiviral condition and activity of natural killers
For the purpose of a preliminary estimate of the therapeutic potential of domestic recombinant alpha/sub 2/-component of human leukocytic interferon (rl) in vitro tests, the authors studied its ability to induce development of antiviral condition in diploid culture of human embryo fibroblasts and to activate the cytolytic effect of natural killers in relation to tumor cells, of the K-562 leukemia line and cells of lung adenocarcinoma. The authors used a medicinal form of rL which was derived by expression of a reconstructed gene in Escherichia coli cells. Part of the tests were conducted with an analogous preparation synthesized using another producer, Pseudomonas sp). The biological effect of both preparations was the same. For comparison, a natural preparation was used in all tests: human leukocytic interferon for injection, II(le). The authors studied activity of natural killers in a fraction of mononuclears isolated from blood of essentially healthy donors and from cancer patients. Cells were incubated for 2 h with various concentrations of interferons, then combined in a ratio of 25-50:1 with target cells labeled with /sup 51/Cr. Cytotoxic reaction was conducted for 4 (4-CTR) or 18 h (18-CTR) at 37/sup 0/C. Natural killers could thus be divided into two subpopulations: killer (4-CTR) and cytotoxic (18-CTR) cells. In preliminary tests, both preparations possessed the ability to active natural killers. The effective concentration for rL was within the limits of 1000-2000 IU/ml, and 50-200 Iu/ml for Le. The data on activation of natural killers in 16 oncological patients (primarily with lung cancer), the authors established that both rL and Le induced activation of natural killers in the overwhelming majority of cases in relation to K-562 target cells and adenocarcinomas of the lung.
- Research Organization:
- N. F. Gamalei Scientific-Research Institute of Epidemiology and Microbiology, Moscow, USSR
- OSTI ID:
- 5856242
- Journal Information:
- Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 284:1-6; Other Information: Translated from Dokl. Akad. Nauk SSSR; 284: No. 6, 1514-1516(Oct 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INTERFERON
BIOLOGICAL EFFECTS
BIOSYNTHESIS
LEUKOCYTES
METABOLIC ACTIVATION
TUMOR CELLS
CELL KILLING
RADIOASSAY
ANTI-INFECTIVE AGENTS
ANTIMITOTIC DRUGS
CARCINOMAS
CELL CULTURES
CHROMIUM 51
DIPLOIDY
EMBRYOS
ESCHERICHIA COLI
FIBROBLASTS
LABELLED COMPOUNDS
LABELLING
LEUKEMIA
MAN
PSEUDOMONAS
RECOMBINANT DNA
RESPIRATORY TRACT CELLS
ANIMAL CELLS
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LYMPHOKINES
MAMMALS
MATERIALS
MICROORGANISMS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
PRIMATES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques