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Title: Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population

Abstract

The use of antibodies to detect chemical carcinogen-induced DNA damage involves quantitative determination and morphological localization utilizing antisera specific for carcinogen-DNA adducts. In recent years a large number of polyclonal and monoclonal antisera have been produced against individual adducts, and modified DNAs with addition products varying in structure from ethyl and methyl groups to aromatic amines, polycyclic aromatic hydrocarbons, aflatoxins, and platinum-ammine complexes. This review focuses on data generated during the past 3 yr utilizing this immunotechnology as a tool to probe mechanisms of carcinogen-DNA interactions in various model systems and in the human population. Areas discussed in depth include quantitative and morphological studies involving detection of 2-acetylaminofluorene-DNA adducts in rat liver, O/sup 6/-ethyl and O/sup 6/-methyl deoxyguanosine adducts in rat brain, benzo(a)pyrene-DNA adducts in mouse skin and cis-diamminedichloroplatinum (II)-DNA adducts in peripheral nucleated blood cells of cancer patients.

Authors:
Publication Date:
Research Org.:
National Cancer Inst., Bethesda, MD
OSTI Identifier:
5856133
Resource Type:
Journal Article
Journal Name:
Environ. Manage.; (United States)
Additional Journal Information:
Journal Volume: 6:6
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOGENS; RADIOIMMUNOASSAY; DNA ADDUCTS; IMMUNE SERUMS; IMMUNOLOGY; IODINE ISOTOPES; BENZOPYRENE; BRAIN; CONDENSED AROMATICS; LEUKOCYTES; LIVER; MAN; NUCLEOSIDES; PLATINUM COMPOUNDS; RATS; SKIN; ADDUCTS; ANIMALS; AROMATICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CENTRAL NERVOUS SYSTEM; DIGESTIVE SYSTEM; GLANDS; HYDROCARBONS; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; NERVOUS SYSTEM; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PRIMATES; RADIOASSAY; RIBOSIDES; RODENTS; TRACER TECHNIQUES; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Poirier, M C. Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population. United States: N. p., 1984. Web. doi:10.1002/em.2860060615.
Poirier, M C. Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population. United States. https://doi.org/10.1002/em.2860060615
Poirier, M C. 1984. "Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population". United States. https://doi.org/10.1002/em.2860060615.
@article{osti_5856133,
title = {Use of carcinogen-DNA adduct antisera for quantitation and localization of genomic damage in animal models and the human population},
author = {Poirier, M C},
abstractNote = {The use of antibodies to detect chemical carcinogen-induced DNA damage involves quantitative determination and morphological localization utilizing antisera specific for carcinogen-DNA adducts. In recent years a large number of polyclonal and monoclonal antisera have been produced against individual adducts, and modified DNAs with addition products varying in structure from ethyl and methyl groups to aromatic amines, polycyclic aromatic hydrocarbons, aflatoxins, and platinum-ammine complexes. This review focuses on data generated during the past 3 yr utilizing this immunotechnology as a tool to probe mechanisms of carcinogen-DNA interactions in various model systems and in the human population. Areas discussed in depth include quantitative and morphological studies involving detection of 2-acetylaminofluorene-DNA adducts in rat liver, O/sup 6/-ethyl and O/sup 6/-methyl deoxyguanosine adducts in rat brain, benzo(a)pyrene-DNA adducts in mouse skin and cis-diamminedichloroplatinum (II)-DNA adducts in peripheral nucleated blood cells of cancer patients.},
doi = {10.1002/em.2860060615},
url = {https://www.osti.gov/biblio/5856133}, journal = {Environ. Manage.; (United States)},
number = ,
volume = 6:6,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1984},
month = {Sun Jan 01 00:00:00 EST 1984}
}