Chemical and biological consequences of the radioactive decay of iodine-125 in plasmid DNA
Doubly labeled (U-/sup 14/C, 5-/sup 125/I)iododeoxycytidine (IdC) triphosphate was synthesized and incorporated enzymatically into defined positions of the plasmid pBR322. After storage under various conditions, the stable end products were analyzed using radio-GC, radio-HPLC, and electron microscopy. In addition, solutions of /sup 14/C-IdC-labeled DNA containing Na/sup 125/I as an internal radiation source were studied to investigate the influence of internal radiolysis. Transmutation of the covalently bound /sup 125/I leads to complete destruction of the labeled nucleotide, giving rise to /sup 14/CO/sub 2/ and /sup 14/CO as major products. Electron microscopy studies reveal that decay-induced double strand breaks (dsb) occur both at the site of decay and in areas as far as hundreds of base pairs apart from that site. Number and distribution of the breaks is strongly dependent on solvent and DNA configuration. A direct correlation exists between the extent of fragmentation of the nucleotide and the mean number of dsb.
- Research Organization:
- Kernforschungsanlage, Juelich, West Germany
- OSTI ID:
- 5854809
- Journal Information:
- Radiat. Res.; (United States), Vol. 101:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
RADIOLYSIS
NUCLEOSIDES
DOUBLE LABELLING
STRAND BREAKS
RADIOINDUCTION
CARBON 14 COMPOUNDS
DNA POLYMERASES
ESCHERICHIA COLI
EXPERIMENTAL DATA
GAS CHROMATOGRAPHY
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
ORGANIC IODINE COMPOUNDS
PLASMIDS
SODIUM IODIDES
TRANSMISSION ELECTRON MICROSCOPY
ALKALI METAL COMPOUNDS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ENZYMES
HALIDES
HALOGEN COMPOUNDS
INFORMATION
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MICROORGANISMS
MICROSCOPY
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHOSPHORS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOISOTOPES
RIBOSIDES
SEPARATION PROCESSES
SODIUM COMPOUNDS
TRANSFERASES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)