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Title: Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]

Abstract

Growth of cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, in the presence of cadmium is inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. Cell growth and phytochelatin synthesis are restored to cells treated with buthionine sulfoximine by the addition of glutathione to the medium. Glutathione stimulates the accumulation of phytochelatins in cadmium treated cells, indicating that availability of glutathione can limit synthesis of these peptides. Exogenous glutathione causes a disproportionate increase in the level of smaller phytochelatins, notably ({gamma}-Glu-Cys){sub 2}-Gly. In the presence of buthionine sulfoximine and glutathione, phytochelatins that are produced upon exposure to cadmium incorporate little ({sup 35}S)cysteine, indicating that these peptides are probably not synthesized by sequential addition of cysteine and glutamate to glutathione.

Authors:
; ;  [1]
  1. Purdue Univ., West Lafayette, IN (USA)
Publication Date:
OSTI Identifier:
5852811
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 93:2; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CADMIUM; BIOLOGICAL EFFECTS; GLUTATHIONE; BIOCHEMICAL REACTION KINETICS; TOMATOES; PHYSIOLOGY; CYSTEINE; ENZYME INHIBITORS; PEPTIDES; PLANT CELLS; SULFUR 35; TRACER TECHNIQUES; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; ELEMENTS; EVEN-ODD NUCLEI; FOOD; FRUITS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; METALS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; POLYPEPTIDES; PROTEINS; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; SULFUR ISOTOPES; THIOLS; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Mendum, M L, Gupta, S C, and Goldsbrough, P B. Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]. United States: N. p., 1990. Web. doi:10.1104/pp.93.2.484.
Mendum, M L, Gupta, S C, & Goldsbrough, P B. Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]. United States. https://doi.org/10.1104/pp.93.2.484
Mendum, M L, Gupta, S C, and Goldsbrough, P B. 1990. "Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]". United States. https://doi.org/10.1104/pp.93.2.484.
@article{osti_5852811,
title = {Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]},
author = {Mendum, M L and Gupta, S C and Goldsbrough, P B},
abstractNote = {Growth of cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, in the presence of cadmium is inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. Cell growth and phytochelatin synthesis are restored to cells treated with buthionine sulfoximine by the addition of glutathione to the medium. Glutathione stimulates the accumulation of phytochelatins in cadmium treated cells, indicating that availability of glutathione can limit synthesis of these peptides. Exogenous glutathione causes a disproportionate increase in the level of smaller phytochelatins, notably ({gamma}-Glu-Cys){sub 2}-Gly. In the presence of buthionine sulfoximine and glutathione, phytochelatins that are produced upon exposure to cadmium incorporate little ({sup 35}S)cysteine, indicating that these peptides are probably not synthesized by sequential addition of cysteine and glutamate to glutathione.},
doi = {10.1104/pp.93.2.484},
url = {https://www.osti.gov/biblio/5852811}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 93:2,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 1990},
month = {Fri Jun 01 00:00:00 EDT 1990}
}