Measurement of nitrogen fixation by soybean in the field using the ureide and natural sup 15 N abundance methods. [Glycine max (L. )]
- New South Wales Agriculture Fisheries, Tamworth (Australia)
- CSIRO Division of Plant Industry, Canberra (Australia)
Nitrogen fixation by field-grown soybean (Glycine max (L.) Merrill) was assessed by the natural {sup 15}N abundance and ureide methods. The field sites (five) and genotypes (six, plus two levels of inoculation on Bragg) were chosen to provide a range of proportions of plant N derived from nitrogen fixation (P). Genotypes K466, K468, nts 1007, and nts 1116 and Davis were included on the basis of their reported tolerance of the suppressive effects of nitrate on nodulation and nitrogen fixation. Bragg was included as a nitrate-sensitive genotype. Seeds of all genotypes were inoculated at sowing with Bradyrhizobium japonicum CB1809 (USDA 136). Amounts of nitrate in the soil profile (0-1.2 meter depth) at sowing ranged from 70 (site 3) to 278 kilograms per hectare (site 5), resulting in large effects on plant nodulation, on the {delta}{sup 15}N values of nodulated plants, on the relative abundance of ureide-N in vacuum-extracted sap (VES) and stem extracts, and finally on the estimates of P. There was no relationship between amount of soil nitrate at sowing and the {delta}{sup 15}N of the plant-available soil N. Correlation matrices of the measured and calculated parameters indicated generally weak correlations between crop growth (dry matter and N) and the parameters of symbiotic activity (nodule weight, {delta}{sup 15}N, relative ureide-N); correlations were strong and highly significant between nodulation and the measures of nitrogen fixation. Estimates of P ranged between 0 and 68% ({delta}{sup 15}N) and between 6 and 56% (ureide) and were highly correlated.
- OSTI ID:
- 5852778
- Journal Information:
- Plant Physiology; (USA), Vol. 93:2; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCINE HISPIDA
NITROGEN FIXATION
GENOTYPE
MEASURING METHODS
NITROGEN 15
PLANT GROWTH
RHIZOBIUM
SOILS
TRACER TECHNIQUES
BACTERIA
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PLANTS
STABLE ISOTOPES
551001* - Physiological Systems- Tracer Techniques