Measurement of human tissue-type plasminogen activator by a two-site immunoradiometric assay
- Univ. of Leuven, Belgium
A two-site immunoradiometric assay for human extrinsic (tissue-type) plasminogen activator was developed by using rabbit antibodies raised against plasminogen activator purified from human melanoma cell culture fluid. Samples of 100 ..mu..l containing 1 to 100 ng/ml plasminogen activator were incubated in the wells of polyvinyl chloride microtiter plates coated with antibody. The amount of bound extrinsic plasminogen activator was quantitated by the subsequent binding of /sup 125/I-labeled affinospecific antibody. The mean level of plasma samples taken at rest was 6.6 +/- 2.9 ng/ml (n = 54). This level increased approximately threefold by exhaustive physical exercise, venous occlusion, or infusion of DDAVP. Extrinsic plasminogen activator in plasma is composed of a fibrin-adsorbable and active component (1.9 +/- 1.1 ng/ml, n = 54, in resting conditions) and an inactive component that does not bind to a fibrin clot (probably extrinsic plasminogen activator-proteinase inhibitor complexes). The fibrin-adsorbable fraction increased approximately fivefold to eightfold after physical exercise, venous occlusion, or DDAVP injections. Potential applications of the immunoradiometric assay are illustrated by the measurement of extrinsic plasminogen activator in different tissue extracts, body fluids, and cell culture fluids and in oocyte translation products after injection with mRNA for plasminogen activator.
- OSTI ID:
- 5851746
- Journal Information:
- J. Lab. Clin. Med.; (United States), Vol. 101:2
- Country of Publication:
- United States
- Language:
- English
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FIBRINOLYTIC AGENTS
RADIOIMMUNOASSAY
PLASMINOGEN
METABOLIC ACTIVATION
BIOCHEMICAL REACTION KINETICS
BLOOD PLASMA
COMPLEXES
EXERCISE
FIBRINOLYSIS
IODINE 125
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
PROTEOLYSIS
RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics