Incorporation of cytokinins into DNA of wheat seedlings
After incubation of wheat seedlings with (/sup 3/H)benzylaminopurine or (8-/sup 3/H)zeathin appreciable radioactivity was found in the purine bases of DNA, mainly in adenine. Moreover, as a result of acid hydrolysis labeled cytokinins were isolated from labeled highly purified preparations of the total DNA of wheat seedlings. Their radioactivity constituted 5-10% of the radioactivity of the adenine bases in the DNA. After mild acid hydrolysis (/sup 3/H)benzylaminopurine and (/sup 3/H)zeathin were found in the deoxyribonucleoside fraction obtained from cytokinin-labeled DNA. Thus, different cytokinins (N/sup 6/-substituted adenine derivatives) are covalently incorporated into the newly synthesized DNA of plants.
- Research Organization:
- Lomonosov Moscow State Univ., Moscow (Russian Federation)
- OSTI ID:
- 5851573
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:2; Other Information: Translated from Biokhimiya; 51: No. 2, 321-327(Feb 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
ACID HYDROLYSIS
TRACER TECHNIQUES
KININS
METABOLISM
PURINES
SEEDS
CYTOCHEMISTRY
ADENINES
BIOSYNTHESIS
CELL DIFFERENTIATION
CHROMATOGRAPHY
ENZYME INHIBITORS
LABELLING
METHYLATION
NUCLEOTIDES
PLANT CELLS
RADIOACTIVITY
RADIONUCLIDE KINETICS
RNA
TRANSCRIPTION
TRITIUM COMPOUNDS
WHEAT
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOCHEMISTRY
CEREALS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DRUGS
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
SEPARATION PROCESSES
SOLVOLYSIS
SYNTHESIS
550501* - Metabolism- Tracer Techniques
550401 - Genetics- Tracer Techniques
550301 - Cytology- Tracer Techniques