Studies of the expression of the cytochrome P450IA, P450IIB, and P450IIC gene family in extrahepatic and hepatic tissues
- Johannes Gutenberg Univ., Mainz (West Germany)
The authors have studied the expression of three P-450 gene subfamilies in hepatic and extrahepatic tissues using the sensitive RNAse A protection assay. Members of the P450IA subfamily, which encodes the major methylcholanthrene-inducible cytochromes P-450, were found to be not expressed in extrahepatic tissues of untreated animals, raising the question whether these P-450 play a role in the metabolism of carcinogens in unexposed individuals. In contrast, members of the P450IIB family, some of which encode the major phenobarbital-inducible cytochromes P-450, were found to be expressed in some extrahepatic tissues of untreated rats and here most notably in the lung and in sebaceous glands. members of the P450IIC family, which encode some constitutively expressed cytochromes P-450, were found to be expressed exclusively in the liver.
- OSTI ID:
- 5848620
- Journal Information:
- Environmental Health Perspectives; (USA), Vol. 88; ISSN 0091-6765
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CYTOCHROME OXIDASE
GENE REGULATION
XENOBIOTICS
METABOLISM
3-METHYLCHOLANTHRENE
BRAIN
CHEMICAL ACTIVATION
GENES
INTESTINES
LIVER
LUNGS
MESSENGER-RNA
RNA-ASE
TESTES
AROMATICS
BODY
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
ENZYMES
ESTERASES
GASTROINTESTINAL TRACT
GLANDS
GONADS
HAEM DEHYDROGENASES
HYDROCARBONS
HYDROLASES
MALE GENITALS
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHODIESTERASES
POLYCYCLIC AROMATIC HYDROCARBONS
RESPIRATORY SYSTEM
RNA
560300* - Chemicals Metabolism & Toxicology