A concerted approach to the study of the aneuploidogenic properties of two chelating agents (EDTA and NTA) in the germ and somatic cell lines of Drosophila and the mouse

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850150406· OSTI ID:5848351

Zordan, M; Russo, A; Costa, R; Bianco, N; Beltrame, C; Levis, A G ^[1]

---

1. Univ. of Padova (Italy)

The genetic effects of nitrilotriacetic acid (NTA) and ethylenedinitrilotetraacetic acid (EDTA), two widely used chelating agents, were investigated by using a somatic mutation and recombination test (SMART) after treatment of larvae and the FIX test for aneuploidy after treatment of adult female Drosophila melanogaster. Chloral hydrate (CH) and 5-fluorodeoxyuridine (FdUr) were used as positive controls. Effectively absorbed amounts of the test compounds assayed in Drosophila were estimated at the single fly level by a method using {sup 3}H-leucine. NTA and EDTA were also assayed in tests for aneuploidy based on chromosome counting in mouse germ and somatic cells. The authors previously showed that NTA was able to induce aneuploidy in the germ cells of both Drosophila and the mouse when tested at the exposure levels of 5 {times} 10{sup {minus}2} M and 275 mg per kg body weight, respectively. In the present experiments, EDTA was assayed at 2.5 {times} 10{sup {minus}2} M and 7.5 {times} 10{sup {minus}3} M in the FIX test adopting a three-stage brooding scheme. Significant increases in chromosomal loss were observed int he second brook and in the combined three-brook total for both exposure levels of EDTA. The previously observed induction of germ cell aneuploidy by NTA was confirmed in the present experiments on a different strain of mice. These results compared and discussed with reference to the characteristics of the different test systems used and to the different chelating properties of NTA and EDTA.

• OSTI ID: 5848351
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Vol. 15:4; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
CELL PROLIFERATION
EDTA
GENETIC EFFECTS
NTA
ANEUPLOIDY
CHELATING AGENTS
DROSOPHILA
GENE RECOMBINATION
LEUCINE
MICE
SOMATIC MUTATIONS
SPERMATOCYTES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DIPTERA
FLIES
FRUIT FLIES
GERM CELLS
INSECTS
INVERTEBRATES
MAMMALS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology