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Title: Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites

Abstract

We have observed electron dense deposits dependent on incubation of aldehyde-fixed tissues with lead ions within synaptic vesicles of several types of neurons that differ in the neurotransmitters utilized and in the secretory granules of the adrenal medulla. Evidently, vesicle components that can interact with lead ions are widespread. A plausible explanation for the occurrence of the deposits is the presence of anionic binding sites within the vesicles. This would agree well with other biochemical, cytochemical, and immunocytochemical evidence, such as that indicating the presence of sulfated macromolecules in certain synaptic vesicles. Anionic binding sites could play significant roles by participating in processes such as Ca/sup 2 +/ storage, stabilization of pH gradients, or the control of osmotic phenomena.

Authors:
; ; ;
Publication Date:
Research Org.:
Columbia Univ., New York, NY
OSTI Identifier:
5844757
Resource Type:
Journal Article
Journal Name:
J. Neurobiol.; (United States)
Additional Journal Information:
Journal Volume: 18:5
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEAD; RECEPTORS; UPTAKE; ANIONS; CEREBRAL CORTEX; ELECTRONS; MICE; NERVE CELLS; RATS; ANIMAL CELLS; ANIMALS; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CEREBRUM; CHARGED PARTICLES; ELEMENTARY PARTICLES; ELEMENTS; FERMIONS; IONS; LEPTONS; MAMMALS; MEMBRANE PROTEINS; METALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SOMATIC CELLS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Sulzer, D, Piscopo, I, Ungar, F, and Holtzman, E. Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites. United States: N. p., 1987. Web. doi:10.1002/neu.480180507.
Sulzer, D, Piscopo, I, Ungar, F, & Holtzman, E. Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites. United States. https://doi.org/10.1002/neu.480180507
Sulzer, D, Piscopo, I, Ungar, F, and Holtzman, E. 1987. "Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites". United States. https://doi.org/10.1002/neu.480180507.
@article{osti_5844757,
title = {Lead-dependent deposits in diverse synaptic vesicles: suggestive evidence for the presence of anionic binding sites},
author = {Sulzer, D and Piscopo, I and Ungar, F and Holtzman, E},
abstractNote = {We have observed electron dense deposits dependent on incubation of aldehyde-fixed tissues with lead ions within synaptic vesicles of several types of neurons that differ in the neurotransmitters utilized and in the secretory granules of the adrenal medulla. Evidently, vesicle components that can interact with lead ions are widespread. A plausible explanation for the occurrence of the deposits is the presence of anionic binding sites within the vesicles. This would agree well with other biochemical, cytochemical, and immunocytochemical evidence, such as that indicating the presence of sulfated macromolecules in certain synaptic vesicles. Anionic binding sites could play significant roles by participating in processes such as Ca/sup 2 +/ storage, stabilization of pH gradients, or the control of osmotic phenomena.},
doi = {10.1002/neu.480180507},
url = {https://www.osti.gov/biblio/5844757}, journal = {J. Neurobiol.; (United States)},
number = ,
volume = 18:5,
place = {United States},
year = {Tue Sep 01 00:00:00 EDT 1987},
month = {Tue Sep 01 00:00:00 EDT 1987}
}