Assimilation of /sup 14/CO/sub 2/ and /sup 14/C sucrose by citrus fruit tissues
Assimilation and metabolism of /sup 14/CO/sub 2/ was compared to that of (U-/sup 14/C) sucrose in young grapefruit (ca 25 mm diameter) to determine their respective roles in fruit growth. Fixation of /sup 14/CO/sub 2/ by isolated fruit tissues during 10 min in light exceeded that in dark by 2- to 30-fold depending on tissue content of chlorophyll. Greatest apparent photosynthesis occurred in outer green peel, but green juice tissues assimilated more than did adjoining inner peel tissue. In the dark, juice tissues incorporated 2.5-fold more /sup 14/CO/sub 2/ than any other tissue. Neutral sugars accounted for a smaller proportion and organic acids, a greater proportion, of the /sup 14/C-assimilates in interior peel and juice tissues. These data suggest more extensive production of organic acids from /sup 14/CO/sub 2/ in tissues isolated from the fruit interior. In contrast, little difference among tissues was evident in extent of organic- and amino-acid production from exogenous (U-/sup 14/C) sucrose. A small area of cuticle on whole fruit was replaced by a filter disc impregnated with radiolabeled sucrose and incubated for 16 h. Thus, carbon derived from CO/sub 2/ assimilation by fruit appears to be partitioned differently than that derived from sucrose.
- Research Organization:
- Univ. of Florida, Gainesville
- OSTI ID:
- 5842027
- Report Number(s):
- CONF-8707108-; TRN: 88-001673
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GRAPEFRUITS
CARBON DIOXIDE FIXATION
METABOLISM
ORGANIC ACIDS
BIOSYNTHESIS
SACCHAROSE
AMINO ACIDS
CARBON 14 COMPOUNDS
CHLOROPHYLL
PLANT GROWTH
TRACER TECHNIQUES
VISIBLE RADIATION
CARBOHYDRATES
CARBOXYLIC ACIDS
DISACCHARIDES
ELECTROMAGNETIC RADIATION
FOOD
FRUITS
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
RADIATIONS
SACCHARIDES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques