Characteristics of enzyme hydrolyzing natural covalent bond between RNA and protein VPg of encephalomyocarditis virus
The isolation and a preliminary characterization of the enzyme specifically hydrolyzing the phosphodiester bond between protein VPg and the RNA of encephalomyocarditis virus was the goal of the present investigation. The enzyme was isolated from a salt extract of Krebs II mouse ascites carcinoma cells by ion-exchange and affinity chromatography. It was found that the enzyme actually specifically cleaves the covalent bond between the RNA and protein, however, the isolation procedure does not free the enzyme from impurities which partially inhibit it. The enzyme cleaves the RNA-protein VPg complex of polio virus at a high rate, it is completely inactivated at 55/sup 0/C, and is partially inhibited by EDTA.
- Research Organization:
- Lomonosov Moscow State Univ., Moscow (Russian Federation)
- OSTI ID:
- 5841962
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:2; Other Information: Translated from Biokhimiya; 51: No. 2, 249-259(Feb 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHOSPHODIESTERASES
ENZYME ACTIVITY
PROTEINS
ENZYMATIC HYDROLYSIS
RADIOENZYMATIC ASSAY
RNA
ASCITES TUMOR CELLS
BIOCHEMISTRY
CHEMICAL BONDS
COVALENCE
ENCEPHALITIS
IODINE 125
KREBS CYCLE
LABELLED COMPOUNDS
LABELLING
MICE
MYOCARDIUM
NUCLEOTIDES
PHOSPHORUS 32
RADIOACTIVITY
TRITIUM COMPOUNDS
TYROSINE
VIRUSES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
HEART
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
LYSIS
MAMMALS
MICROORGANISMS
MUSCLES
NERVOUS SYSTEM DISEASES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RODENTS
SOLVOLYSIS
TUMOR CELLS
VERTEBRATES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques