Proteoglycan metabolism associated with mouse metanephric development: morphologic and biochemical effects of beta-D-xyloside
Morphology and de novo incorporation of (/sup 35/S)sulfate into proteoglycans were studied in fetal mouse kidneys at the onset of organogenesis. Branching morphogenesis and nephron development in organ culture and in vivo were associated with de novo synthesis of chondroitin-SO/sub 4/ and heparan-SO/sub 4/ proteoglycans. The role of proteoglycan metabolism in metanephrogenesis was then studied by analysis of the effects of p-nitrophenyl-beta-D-xylopyranoside (beta-D-xyloside) on renal development and proteoglycan metabolism. Incubation of fetal kidneys in beta-D-xyloside at concentrations of 1.0 and 0.5 mM, but not at 0.1 mM, caused inhibition of ureteric branching and markedly diminished synthesis of a large Mr 2.0 X 10(6) Da chondroitin-SO/sub 4/ proteoglycan. Incorporation of (/sup 35/S)sulfate was stimulated at all beta-D-xyloside concentrations, reflecting synthesis of xyloside initiated dermatan-/sup 35/SO/sub 4/ chains. In contrast to dramatic effects on chondroitin-SO/sub 4/ synthesis and ureteric branching, beta-D-xyloside had no effect on heparan-SO/sub 4/ synthesis or on development of the glomerulus and glomerular basement membrane. We thus characterize the proteoglycans synthesized early in the course of renal organogenesis and describe observations which suggest an association between metabolism of chondroitin-SO/sub 4/ proteoglycan and development of the ureter.
- Research Organization:
- Univ. of Minnesota, Minneapolis
- OSTI ID:
- 5841743
- Journal Information:
- Dev. Biol.; (United States), Vol. 123:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOPROTEINS
METABOLISM
GLYCOSIDES
BIOLOGICAL EFFECTS
KIDNEYS
CELL DIFFERENTIATION
FETUSES
INHIBITION
MICE
ONTOGENESIS
SULFATES
SULFUR 35
TISSUE CULTURES
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques