Synthesis of. beta. -D-glucan in vitro: HPLC separation of oligosaccharides from enzymic digests of glucans synthesized by Golgi apparatus and UDP-glucose

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5841376

Gibeaut, D M; Carpita, N C ^[1]

---

1. Purdue Univ., West Lafayette, IN (USA)

Anion exchange HPLC (Dionex) resolves {beta}-D-glucose oligosaccharides which vary in (1{yields}3) and (1{yields}4) linkage structure. The products from incubation of UDPG and cellular membranes from maize enriched for Golgi apparatus, ER, and plasma membrane are digested with glucan hydrolases specific for either (1{yields}3) or (1{yields}4) linkages and one which requires a specific (1{yields}3){beta}-D-glc-(1{yields}4){beta}-D-glc sequence. Two oligomers released by the latter enzyme are diagnostic of cereal grass mixed-linkage {beta}-D-glucan and are used as standards to examine reaction conditions for synthesis of this polymer with radioactive substrates. Our preliminary data indicate that formation of specific sequences of {beta}-D-glucan is increased by high concentrations of UDPG. Other radioactive oligomers are present in greater quantity than those from authentic mixed-linkage glucan and their linkage structure is now under investigation.

• OSTI ID: 5841376
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
STARCH
BIOSYNTHESIS
UDPG
BIOLOGICAL EFFECTS
CEREALS
ENDOPLASMIC RETICULUM
HYDROLASES
MAIZE
MEMBRANES
ORGANOIDS
CARBOHYDRATES
CELL CONSTITUENTS
ENZYMES
GLYCOSIDES
GRAMINEAE
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANTS
POLYSACCHARIDES
REAGENTS
SACCHARIDES
SYNTHESIS
550500* - Metabolism