The induction of heat tolerance in black spruce seedlings
- Univ. of Toronto, Ontario (Canada)
- OMNR, Maple, Ontario (Canada)
Heat conditioning of black spruce seedlings at 38{degree}C drastically reduced heat-induced damage of seedlings exposed to 49-52{degree}C. The pattern of protein synthesis during heat conditioning and heat stress was studied. Seedlings were in vivo labeled with ({sup 35}S)-methionine and protein fractions (soluble, mitochonrial and microsomal) were isolated from roots and needles. Proteins were separated by SDS-PAGE and identified by stainign and autoradiography. Several heat-shock proteins (HSP) were produced during heat conditioning and heat shock, but only few de novo synthesized proteins were specific to the heat conditioning treatments. Experiments used to distinguish between the putative HSP from those proteins specifically associated with the heat conditioning process will be presented and discussed.
- OSTI ID:
- 5841292
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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PROTEINS
TEMPERATURE DEPENDENCE
BIOLOGICAL EFFECTS
IN VIVO
SEEDLINGS
SPRUCES
SULFUR 35
SULFUR COMPOUNDS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CONIFERS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ORGANIC COMPOUNDS
PINOPHYTA
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
TREES
550501* - Metabolism- Tracer Techniques