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Title: Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species

Abstract

Pseudomonas PG-1 cultivated on pristane produced in good amount a heat-stable polymeric substance which showed strong hydrocarbon emulsifying and solubilizing properties. The substance was isolated in crude form and was found to contain 34% protein, 16% carbohydrate, and 40% lipid. The hydrocarbon solubilizing activity of the isolate was strongly inhibited by EDTA but the chelating agent had no effect on the hydrocarbon emulsifying activity. Both activities of the isolate were strongly inhibited by chymotrypsin treatment indicating the importance of the protein moiety for its activity. Hydrocarbon solubilization by the isolate showed a certain degree of specificity to pristane in modest agitation generally used in microbial cultivation, but this specificity was lost by vigorous agitation in a Waring blender. It was proposed that in the first case, solubilization was effected by a solubilizing factor specific to pristane, whereas in the latter ease, nonspecific solubilization occurred due to the action of the emulsifying factor. The rate of pristane solubilization by heat-treated culture broth under the conditions of agitation used in cultivation (rotary shaker, 120 rpm) was found to be ca. 750 mg L/sup -1/h/sup -1/ which was much larger than the maximal pristane uptake rate of 170 mg L/sup -1/h/sup -1/ observedmore » during microbial growth on the substrate. It was concluded that hydrocarbon solubilization could satisfactorily account for the substrate uptake and growth.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Biochemistry Division, Regional Research Laboratory, Jorhat 785006, Assam
OSTI Identifier:
5837274
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: XXV:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; HYDROCARBONS; EMULSIFICATION; SOLUBILITY; PSEUDOMONAS; CULTIVATION; CARBOHYDRATES; LIPIDS; PROTEINS; BACTERIA; MICROORGANISMS; ORGANIC COMPOUNDS; 550200* - Biochemistry; 400100 - Analytical & Separations Chemistry

Citation Formats

Reddy, P G, Banuah, J N, Bhagat, S D, Pathak, M G, and Singh, H D. Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species. United States: N. p., 1983. Web. doi:10.1002/bit.260250208.
Reddy, P G, Banuah, J N, Bhagat, S D, Pathak, M G, & Singh, H D. Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species. United States. https://doi.org/10.1002/bit.260250208
Reddy, P G, Banuah, J N, Bhagat, S D, Pathak, M G, and Singh, H D. 1983. "Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species". United States. https://doi.org/10.1002/bit.260250208.
@article{osti_5837274,
title = {Isolation and functional characterization of hydrocarbon emulsifying and solubilizing factors produced by a Pseudomonas species},
author = {Reddy, P G and Banuah, J N and Bhagat, S D and Pathak, M G and Singh, H D},
abstractNote = {Pseudomonas PG-1 cultivated on pristane produced in good amount a heat-stable polymeric substance which showed strong hydrocarbon emulsifying and solubilizing properties. The substance was isolated in crude form and was found to contain 34% protein, 16% carbohydrate, and 40% lipid. The hydrocarbon solubilizing activity of the isolate was strongly inhibited by EDTA but the chelating agent had no effect on the hydrocarbon emulsifying activity. Both activities of the isolate were strongly inhibited by chymotrypsin treatment indicating the importance of the protein moiety for its activity. Hydrocarbon solubilization by the isolate showed a certain degree of specificity to pristane in modest agitation generally used in microbial cultivation, but this specificity was lost by vigorous agitation in a Waring blender. It was proposed that in the first case, solubilization was effected by a solubilizing factor specific to pristane, whereas in the latter ease, nonspecific solubilization occurred due to the action of the emulsifying factor. The rate of pristane solubilization by heat-treated culture broth under the conditions of agitation used in cultivation (rotary shaker, 120 rpm) was found to be ca. 750 mg L/sup -1/h/sup -1/ which was much larger than the maximal pristane uptake rate of 170 mg L/sup -1/h/sup -1/ observed during microbial growth on the substrate. It was concluded that hydrocarbon solubilization could satisfactorily account for the substrate uptake and growth.},
doi = {10.1002/bit.260250208},
url = {https://www.osti.gov/biblio/5837274}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = XXV:2,
place = {United States},
year = {Tue Feb 01 00:00:00 EST 1983},
month = {Tue Feb 01 00:00:00 EST 1983}
}