Detection and comparison of DNA adducts after in vitro and in vivo diesel emission exposures
Development of methodologies to evaluate certain classes of polycyclic aromatic compounds (PAC) detected in complex mixtures to which humans are exposed would greatly improve the diagnostic potential of (32)P-postlabeling analysis. Identification of DNA adduct patterns of specific exposure-related marker adducts would strengthen associations between observed DNA adducts and exposures to different environmental pollutants (e.g., kerosene, cigarette smoke, coke oven, and diesel). Diesel-modified DNA adduct patterns were compared in various in vitro and in vivo rodent model systems and then compared to DNA reactive oxidative and reductive metabolites of 1-nitropyrene. The formation of nitrated-polycyclic aromatic hydrocarbon (nitrated-PAH) DNA adducts, derived from the metabolism of diesel extract constituents, was enhanced relative to other PAH-derived DNA adducts via xanthine oxidase-catalyzed nitroreduction. These adducts were detectable only by the butanol extraction version of the postlabeling analysis. Marker adducts detected in the various test systems presented here will assist in characterizing nuclease-P1-sensitive nitrated PAH adducts in humans.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
- OSTI ID:
- 5837222
- Report Number(s):
- PB-93-228856/XAB; EPA-600/J-93/359
- Resource Relation:
- Other Information: Pub. in Environmental Health Perspectives, Vol. 99, 225-228(Mar 1993)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DIESEL FUELS
ENVIRONMENTAL EXPOSURE
HEALTH HAZARDS
DNA ADDUCTS
DETECTION
MEASURING METHODS
DIAGNOSTIC TECHNIQUES
EXHAUST GASES
GASEOUS WASTES
HUMAN POPULATIONS
IN VITRO
IN VIVO
METABOLITES
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
ADDUCTS
ANIMALS
AROMATICS
DISTILLATES
ENERGY SOURCES
FLUIDS
FOSSIL FUELS
FUELS
GAS OILS
GASES
HAZARDS
HYDROCARBONS
LIQUID FUELS
MAMMALS
ORGANIC COMPOUNDS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
POPULATIONS
VERTEBRATES
WASTES
560300* - Chemicals Metabolism & Toxicology
550400 - Genetics