Collagen metabolism in mouse lung after X irradiation
Collagen and total protein synthesis rates have been determined in the lungs of CBA mice irradiated with single doses of X rays between 8 and 16 Gy. Mice were injected with (/sup 3/H)proline accompanied by a large dose of unlabeled proline, and synthesis rates were measured at 2-month intervals from 8 to 31 weeks after irradiation. At 2 months after radiation treatment, collagen and total protein synthesis rates were significantly depressed but they had recovered by 4 months. By 6 months collagen synthesis rates had increased above control in a dose-dependent manner, so that in the 14-Gy dose group the fractional synthesis rate for collagen was 4.6 times higher than in control mice as measured by incorporation of (/sup 3/H)proline. However, a significant net accumulation of collagen was seen only in the lungs of the highest dose group at 31 weeks, as indicated by total hydroxyproline measurements. There was a slight increase in the ratio of types I and III collagen. Late radiation damage in the CBA mouse lung is characterized by increased collagen metabolism, which may or may not lead to a net accumulation of collagen.
- Research Organization:
- Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, England
- OSTI ID:
- 5836714
- Journal Information:
- Radiat. Res.; (United States), Vol. 111:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COLLAGEN
METABOLISM
LUNGS
BIOLOGICAL RADIATION EFFECTS
PROTEINS
BIOSYNTHESIS
DOSE-RESPONSE RELATIONSHIPS
FIBROSIS
HYDROXYPROLINE
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
X RADIATION
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SYNTHESIS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals