Effect of dilution on carboxymethylcellulase and xylanase assays

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260260825· OSTI ID:5833170

Cauchon, N; LeDuy, A

It has been demonstrated that the dilution of samples prior to the carboxymethylcellulase and xylanase assays causes serious discrepancies in the numerical values obtained for the enzyme activities. Even when the sample is assayed with the identical procedure, one could obtain different numerical values of the enzyme activity U depending on how much this sample has been diluted before the enzyme assay. Two crude commercial cellulase samples of Aspergillus niger and Trichoderma viride as well as the culture filtrate of our newly isolated acidophilic fungus have been used for the demonstration. An empirical method for reporting the cellulolytic activity by taking into account this dilution effect is proposed.

• Research Organization: Laval Univ., Sainte-Foy, Quebec
• OSTI ID: 5833170
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 26:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
CELLULASE
CELLULOLYTIC ACTIVITY
XYLANASE
ASPERGILLUS
DILUTION
ENZYMES
ERRORS
MEASURING METHODS
QUALITATIVE CHEMICAL ANALYSIS
TRICHODERMA VIRIDE
CHEMICAL ANALYSIS
ENZYME ACTIVITY
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
PLANTS
TRICHODERMA
550700* - Microbiology
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