Translation of adenovirus 2 late mRNAs microinjected into cultured African green monkey kidney cells

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · J. Virol.; (United States)

OSTI ID:5822857

Richardson, W D; Anderson, C W

Adenovirus 2-infected monkey cells fail to synthesize fiber, a 62,000 M/sub r/ virion polypeptide expressed at late times in productively infected cells. Yet these cells contain fiber mRNA that, after isolation, can be translated in vitro. The reason for the failure of monkey cells to translate fiber mRNA has been approached by microinjecting adenovirus mRNA into the cytoplasm of cultured monkey cells. Late adenovirus 2 mRNA, isolated from infected HeLa cells, was efficiently expressed when microinjected into the African green monkey kidney cell line CV-C. Expressed viral proteins identified by immunoprecipitation included the adenovirus fiber polypeptide. This result demonstrates that the monkey cell translational apparatus is capable of recognizing and expressing functional adenovirus mRNA. Microinjection of late virus mRNA into cells previously infected with wild-type adenovirus 2 failed to increase significantly the yield of infectious virus. 26 references, 2 figures, 1 table.

• Research Organization: National Inst. of Child Health and Human Development, Bethesda, MD
• OSTI ID: 5822857
• Journal Information: J. Virol.; (United States), Vol. 51:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
GROWTH
POLYPEPTIDES
BIOSYNTHESIS
ANIMAL CELLS
CYTOPLASM
EXPERIMENTAL DATA
HELA CELLS
IMMUNE REACTIONS
KIDNEYS
MESSENGER-RNA
MONKEYS
ANIMALS
BODY
CELL CONSTITUENTS
DATA
INFORMATION
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
NUMERICAL DATA
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PEPTIDES
PRIMATES
PROTEINS
RNA
SYNTHESIS
VERTEBRATES
VIRUSES
550200* - Biochemistry