Measurement of anti-IgA antibodies by a two-site immunoradiometric assay
To enable the detection of IgG class, anti-IgA antibodies and to investigate the possible occurrence of IgE class, anti-IgA antibodies, we developed a solid phase immunoradiometric assay, which uses purified IgA coupled covalently to microcrystalline cellulose as an immunosorbent. Radiolabeled, Fc specific anti-IgG and anti-IgE antibodies were used to detect specific aIgA after incubation of test sera or controls with the immunosorbent. IgG-aIgA were detected by the IRA in 100 and 67% of control sera with class specific and limited specificity aIgA. The IRA was sensitive to approximately two ng of class specific IgG-aIgA. IgG-aIgA also were detected by IRA in 7.9% of sera from patients with urticarial transfusion reactions and 73% of sera from patients with ataxia telangiectasia and IgA deficiency. Sera from 50 normal blood donors did not have detectable IgG-aIgA. Tests for IgE-aIgA were negative in all cases, including control sera with class specific IgG-aIgA. We conclude that the IRA is a sensitive and reproducible method for detection of class specific and limited specificity IgG-aIgA, and that IgE-aIgA do not mediate urticarial transfusion reactions.
- OSTI ID:
- 5822116
- Journal Information:
- Transfusion; (United States), Vol. 21:1
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
RADIOIMMUNOASSAY
TRANSFUSIONS
IMMUNE REACTIONS
BIOCHEMISTRY
BLOOD
IMMUNE SERUMS
IMMUNOGLOBULINS
PATIENTS
TELANGIECTASIS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMISTRY
DISEASES
GLOBULINS
ISOTOPE APPLICATIONS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
SKIN DISEASES
THERAPY
TRACER TECHNIQUES
VASCULAR DISEASES
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551001 - Physiological Systems- Tracer Techniques