A simple radiometric in vitro assay for acetylcholinesterase inhibitors
Journal Article
·
· J. Pharm. Sci.; (United States)
A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of (/sup 14/C)carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC50) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.
- Research Organization:
- Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD
- OSTI ID:
- 5821944
- Journal Information:
- J. Pharm. Sci.; (United States), Vol. 72:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ENZYME INHIBITORS
RADIOASSAY
ACETYLCHOLINE
CARBON 14 COMPOUNDS
CHOLINESTERASE
IN VITRO
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLESTERASES
DRUGS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
LABELLED COMPOUNDS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
550201* - Biochemistry- Tracer Techniques
ENZYME INHIBITORS
RADIOASSAY
ACETYLCHOLINE
CARBON 14 COMPOUNDS
CHOLINESTERASE
IN VITRO
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLESTERASES
DRUGS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
LABELLED COMPOUNDS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
550201* - Biochemistry- Tracer Techniques