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Title: A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12

Abstract

Melanocytes preferentially express an mRNA species, Pmel 17, whose protein product cross-reacts with anti-tyrosinase antibodies and whose expression correlates with the melanin content. The authors have now analyzed the deduced protein structure and mapped its chromosomal location in mouse and human. The amino acid sequence deduced from the nucleotide sequence of the Pmel 17 cDNA showed that the protein is composed of 645 amino acids with a molecular weight of 68,600. The Pmel 17 protein contains a putative leader sequence and a potential membrane anchor segment, which indicates that this may be a membrane-associated protein in melanocytes. The deduced protein contains five potential N-glycosylation sites and relatively high levels of serine and threonine. Three repeats of a 26-amino acid motif appear in the middle of the molecule. The human Pmel 17 gene, designated D12S53E, maps to chromosome 12, region 12pter-q21; and the mouse homologue, designated D12S53Eh, maps to the distal region of mouse chromosome 10, a region also known to carry the coat color locus si (silver).

Authors:
; ; ;  [1];  [2]; ; ;  [3]; ;  [4]
  1. Indiana Univ.-Purdue Univ., Indianapolis (United States)
  2. National Inst. of Health, Bethesda, MD (United States)
  3. National Cancer Inst., Frederick, MD (United States)
  4. Yale Univ., New Haven, CT (United States)
Publication Date:
OSTI Identifier:
5821608
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:20; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HETEROCHROMOSOMES; GENETIC MAPPING; PROTEINS; AMINO ACID SEQUENCE; DNA HYBRIDIZATION; DNA SEQUENCING; MELANIN; MESSENGER-RNA; MICE; PIGMENTS; RFLPS; ANIMALS; CHROMOSOMES; HYBRIDIZATION; HYDROXY COMPOUNDS; MAMMALS; MAPPING; MOLECULAR STRUCTURE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RNA; RODENTS; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550400* - Genetics

Citation Formats

Kwon, B S, Chintamaneni, C, Kobayashi, Y, Kim, K K, Kozak, C A, Copeland, N G, Gilbert, D J, Jenkins, N, Barton, D, and Francke, U. A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12. United States: N. p., 1991. Web. doi:10.1073/pnas.88.20.9228.
Kwon, B S, Chintamaneni, C, Kobayashi, Y, Kim, K K, Kozak, C A, Copeland, N G, Gilbert, D J, Jenkins, N, Barton, D, & Francke, U. A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12. United States. https://doi.org/10.1073/pnas.88.20.9228
Kwon, B S, Chintamaneni, C, Kobayashi, Y, Kim, K K, Kozak, C A, Copeland, N G, Gilbert, D J, Jenkins, N, Barton, D, and Francke, U. 1991. "A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12". United States. https://doi.org/10.1073/pnas.88.20.9228.
@article{osti_5821608,
title = {A melanocyte-specific gene, Pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12},
author = {Kwon, B S and Chintamaneni, C and Kobayashi, Y and Kim, K K and Kozak, C A and Copeland, N G and Gilbert, D J and Jenkins, N and Barton, D and Francke, U},
abstractNote = {Melanocytes preferentially express an mRNA species, Pmel 17, whose protein product cross-reacts with anti-tyrosinase antibodies and whose expression correlates with the melanin content. The authors have now analyzed the deduced protein structure and mapped its chromosomal location in mouse and human. The amino acid sequence deduced from the nucleotide sequence of the Pmel 17 cDNA showed that the protein is composed of 645 amino acids with a molecular weight of 68,600. The Pmel 17 protein contains a putative leader sequence and a potential membrane anchor segment, which indicates that this may be a membrane-associated protein in melanocytes. The deduced protein contains five potential N-glycosylation sites and relatively high levels of serine and threonine. Three repeats of a 26-amino acid motif appear in the middle of the molecule. The human Pmel 17 gene, designated D12S53E, maps to chromosome 12, region 12pter-q21; and the mouse homologue, designated D12S53Eh, maps to the distal region of mouse chromosome 10, a region also known to carry the coat color locus si (silver).},
doi = {10.1073/pnas.88.20.9228},
url = {https://www.osti.gov/biblio/5821608}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:20,
place = {United States},
year = {Tue Oct 15 00:00:00 EDT 1991},
month = {Tue Oct 15 00:00:00 EDT 1991}
}