Structure of the propeptide of prothrombin containing the. gamma. -carboxylation recognition site determined by two-dimensional NMR spectroscopy

Journal Article · Tue Oct 15 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00105a004· OSTI ID:5820204

Sanford, D G; Sudmeier, J L; Bachovchin, W W; Kanagy, C; Furie, B C; Furie, B ^[1]

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1. Tufts Univ., Boston, MA (United States)

The propeptides of the vitamin K dependent blood clotting and regulatory proteins contain a {gamma}-carboxylation recognition site that directs precursor forms of these proteins for posttranslational {gamma}-carboxylation. Peptides corresponding to the propeptide of prothrombin were synthesized and examined by circular dichroism (CD) and nuclear magnetic resonance spectroscopy (NMR). CD spectra indicate that these peptides have little or no secondary structure in aqueous solutions but that the addition of trifluoroethanol induces or stabilizes a structure containing {alpha}-helical character. The maximum helical content occurs at 35-40% trifluoroethanol. This trifluoroethanol-stabilized structure was solved by two-dimensional NMR spectroscopy. The NMR results demonstrate that residues {minus}13 to {minus}3 form an amphipathic {alpha}-helix. NMR spectra indicate that a similar structure is present at 5C, in the absence of trifluoroethanol. Of the residues previously implicated in defining the {gamma}-carboxylation recognition site, four residues ({minus}18, {minus}17, {minus}16, and {minus}15) are adjacent to the helical region and one residue ({minus}10) is located within the helix. The potential role of the amphipathic {alpha}-helix in the {gamma}-carboxylation recognition site is discussed.

• OSTI ID: 5820204
• Journal Information: Biochemistry; (United States), Vol. 30:41; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD COAGULATION FACTORS
MOLECULAR STRUCTURE
PEPTIDES
NUCLEAR MAGNETIC RESONANCE
CARBOXYLATION
DICHROISM
HEAVY WATER
PROTHROMBIN
PROTONS
VITAMIN K
AROMATICS
BARYONS
CHEMICAL REACTIONS
COAGULANTS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
QUINONES
RESONANCE
VITAMINS
WATER
550600* - Medicine