Induction of pyrimidine dimers in epidermal DNA of hairless mice by UVB: an action spectrum

Journal Article · Tue Mar 01 00:00:00 EST 1983 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12534010· OSTI ID:5816038

Ley, R D; Peak, M J; Lyon, L L

An action spectrum for the induction of pyrimidine dimers in the epidermis of hairless mice was determined between 288 and 307 nm. The presence of pyrimidine dimers in tritium-labeled DNA extracted from exposed SKH:hairless-1 mouse skin was determined using dimer-specific nucleases from Micrococcus luteus in conjunction with sedimentation of the irradiated DNA in alkaline sucrose gradients. The rate of induction of pyrimidine dimers was maximal at 293 nm. These values were used to propose a UVB transmission curve for mouse epidermis.

• Research Organization: Biology Division, Oak Ridge National Laboratory, Tennessee
• OSTI ID: 5816038
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 80:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
CHEMICAL RADIATION EFFECTS
PYRIMIDINES
DIMERS
MICE
RADIOINDUCTION
SKIN
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA
ANIMALS
AZINES
BODY
CHEMISTRY
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RODENTS
SPECTRA
VERTEBRATES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)